Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting

Journal of Translational Medicine

Valeria Calcaterra⁵  Serena Piccinno⁶  Daniela Ingo²  Melissa Mantelli²  Federico Costanzo³  Erika Andreatta³  Annalisa De Silvestri⁴  Massimo Dominici⁶  Rita Maccario²  Luigi Avolio³  Piero Romano³  Monica Osti³  Antonia Icaro Cornaglia¹  Maria Antonietta Avanzini²  Gloria Pelizzo³ 

[1] Histology and Embryology Unit, Department of Public Health, Experimental Medicine and Forensic, University of Pavia, Pavia, Italy;Immunology and Transplantation Laboratory/Cell Factory/Pediatric Hematology/Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy;Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, 27100, Italy;Biometry and Clinical Epidemiology Unit, Scientific Direction, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy;Pediatric Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy;Laboratory of Cellular Therapies, Department of Medical and Surgical Sciences for Children and Adults, University Hospital of Modena and Reggio, Emilia, Italy

关键词: Regenerative medicine;    Pediatric surgery;    Bone marrow;    Adipose;    Cutaneous wounds;    Mesenchymal stromal cells;   

Others  :  1221469
DOI  :  10.1186/s12967-015-0580-3

 received in 2015-05-11, accepted in 2015-06-24,  发布年份 2015

【 摘 要 】

Objective

Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs).

Methods

Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. When an adequate number of cells (ASCs 10 × 10 ⁶and BM-MSCs 3 × 10 ⁶ , because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. The first was topically treated with cell infusions, the second was used as a control. The intradermal inoculation included autologous or allogeneic ASCs or autologous BM-MSCs. For histological examination, animals were sacrificed and wounds were harvested after 11 and 21 days of treatment.

Results

Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). Topically, ASC inoculation provided more rapid wound healing than BM-MSCs and controls. Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting. ASCs also improved restoration of skin architecture during wound healing.

Conclusion

The use of ASCs may offer a promising solution to treat extended wounds. Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research.

【 授权许可】

   
2015 Pelizzo et al.

【 图 表 】

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