Plasma miRNAs as potential biomarkers of chronic degenerative valvular disease in Dachshunds

BMC Veterinary Research

Michał Jank¹  Marta Parzeniecka-Jaworska²  Magdalena Garncarz²  Magdalena Hulanicka¹ 

[1] Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska str. 159c, Warsaw, 02-776, Poland;Department of Veterinary Diagnostics and Pathology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska str. 159c, Warsaw, 02-776, Poland

关键词: qPCR;    Dog;    ACVIM;    Plasma;    miRNA;    Endocardiosis;    Dachshunds;   

Others  :  1118959
DOI  :  10.1186/s12917-014-0205-8

 received in 2014-05-07, accepted in 2014-08-28,  发布年份 2014

【 摘 要 】

Background

Endocardiosis is the most common heart disease in Dachshunds and is therefore an important cause of cardiac morbidity and death. In recent years we have observed an increasing interest in the development of new genetic and genomic markers of heart disease. The discovery of miRNAs circulating in biofluids such as plasma or serum aroused researchers’ interest in using them as potential biomarkers. In the present study we analysed the expression of 9 miRNAs described in literature as being involved in cardiovascular pathology in the plasma of dogs suffering from endocardiosis.

Results

Expression analysis using the Real-time PCR method revealed that two out of nine miRNAs were significantly downregulated: the expression of miR-30b differed between ACVIM stage B and stage A (control) dogs; the expression of mi-133b differed ACVIM stage C and stage A dogs. 5 miRNAs (miR-125, miR-126, miR-21, miR-29b and miR-30b) showed a trend of downregulation in the ACVIM C group. Levels of miR-423 were the same in healthy and diseased dogs. Expression of miR-208a and 208b was not detected.

Conclusions

miR-30b could be a potential biomarker of ACVIM stage B heart failure in Dachshunds with endocardiosis and miR-133b could be a potential biomarker of ACVIM stage C. The lack of expression or lack of significant changes in expression in 7 miRNAs which are potential biomarkers of heart diseases in humans proves that findings from human medicine are not always directly reflected in veterinary medicine.

【 授权许可】

   
2014 Hulanicka et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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