Extremely low Helicobacter pylori prevalence in North Sulawesi, Indonesia and identification of a Maori-tribe type strain: a cross sectional study

Gut Pathogens

Yoshio Yamaoka³  Paul N Harijanto¹  ᅟ Nasronudin⁴  Suhintam Pusarawati⁴  Indah S Tantular⁴  Miyuki Matsuda⁵  Fumihiko Kawamoto⁵  Yasutoshi Kido⁵  Rumiko Suzuki⁵  Josef Tuda²  Muhammad Miftahussurur⁴ 

[1] Department of Internal Medicine, Public Hospital of Bethesda Tomohon, Minahasa 93562, Indonesia;Department of Parasitology, Faculty of Medicine, Sam Ratulangi University, Manado 95115, Indonesia;Department of Medicine-Gastroenterology, Baylor College of Medicine and Michael E. Debakey Veterans Affairs Medical Center, 2002 Holcombe Blvd, Houston 77030, Texas, USA;Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu-City 879-5593, Oita, Japan

关键词: Indonesia;    Maori;    Helicobacter pylori;   

Others  :  1137561
DOI  :  10.1186/s13099-014-0042-0

 received in 2014-08-03, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

Sulawesi in Indonesia has a unique geographical profile with assumed separation from Sundaland. Studies of Helicobacter pylori in this region are rare due to the region’s rural location and lack of endoscopy equipment. Indirect methods are, therefore, the most appropriate for measuring H. pylori infection in these areas; with the disposable gastric brush test, we can obtain gastric juice as well as small gastric tissue samples for H. pylori culture. We investigated the prevalence of H. pylori infection and evaluated human migration patterns in the remote areas of North Sulawesi.

Methods

We recruited a total of 251 consecutive adult volunteers and 131 elementary school children. H. pylori infection was determined by urine antibody test. A gastric brush test was used to culture H. pylori. We used next-generation and polymerase chain reaction based sequencing to determine virulence factors and multi-locus sequence typing (MLST).

Results

The overall H. pylori prevalence was only 14.3% for adults and 3.8% for children, and 13.6% and 16.7% in Minahasanese and Mongondownese participants, respectively. We isolated a single H. pylori strain, termed -Manado-1. Manado-1 was East Asian type cagA (ABD type), vacA s1c-m1b, iceA1 positive/iceA2 negative, jhp0562-positive/β-(1,3) galT-negative, oipA “on”, and dupA-negative. Phylogenetic analyses showed the strain to be hspMaori type, a major type observed in native Taiwanese and Maori tribes.

Conclusions

Our data support that very low H. pylori infection prevalence in Indonesia. Identification of hspMaori type H. pylori in North Sulawesi may support the hypothesis that North Sulawesi people migrated from north.

【 授权许可】

   
2014 Miftahussurur et al.; licensee BioMed Central Ltd.

【 图 表 】

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