Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant

GigaScience

Alexander Sczyrba²  Andreas Schlüter¹  Alfred Pühler¹  Andreas Albersmeier¹  Anika Winkler¹  Felix Eikmeyer¹  Peter Belmann²  Irena Maus¹  Andreas Bremges² 

[1] Center for Biotechnology, Bielefeld University, Bielefeld 33615, Germany;Faculty of Technology, Bielefeld University, Bielefeld 33615, Germany

关键词: Assembly;    Sequencing;    Metatranscriptomics;    Metagenomics;    Methanogenesis;    Wet fermentation;    Anaerobic digestion;    Biogas;   

Others  :  1221409
DOI  :  10.1186/s13742-015-0073-6

 received in 2015-05-05, accepted in 2015-07-12,  发布年份 2015

【 摘 要 】

Background

The production of biogas takes place under anaerobic conditions and involves microbial decomposition of organic matter. Most of the participating microbes are still unknown and non-cultivable. Accordingly, shotgun metagenome sequencing currently is the method of choice to obtain insights into community composition and the genetic repertoire.

Findings

Here, we report on the deeply sequenced metagenome and metatranscriptome of a complex biogas-producing microbial community from an agricultural production-scale biogas plant. We assembled the metagenome and, as an example application, show that we reconstructed most genes involved in the methane metabolism, a key pathway involving methanogenesis performed by methanogenic Archaea. This result indicates that there is sufficient sequencing coverage for most downstream analyses.

Conclusions

Sequenced at least one order of magnitude deeper than previous studies, our metagenome data will enable new insights into community composition and the genetic potential of important community members. Moreover, mapping of transcripts to reconstructed genome sequences will enable the identification of active metabolic pathways in target organisms.

【 授权许可】

   
2015 Bremges et al.

【 图 表 】

Fig. 1.

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