Components of the dorsal-ventral pathway also contribute to anterior-posterior patterning in honeybee embryos (Apis mellifera)

EvoDevo

Peter K Dearden¹  Nathan J Kenny²  Megan J Wilson³ 

[1] Laboratory for Evolution and Development, Genetics Otago and Gravida, Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand;Current address: Evolution and Development Research Group, Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK;Developmental Biology Laboratory, Department of Anatomy, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand

关键词: Toll;    Honeybee;    Evolution;    Dpp;    Dorsal ventral;    Axis formation;    Apis mellifera;    Anterior posterior;   

Others  :  801888
DOI  :  10.1186/2041-9139-5-11

 received in 2013-10-07, accepted in 2014-01-20,  发布年份 2014

【 摘 要 】

Background

A key early step in embryogenesis is the establishment of the major body axes; the dorsal-ventral (DV) and anterior-posterior (AP) axes. Determination of these axes in some insects requires the function of different sets of signalling pathways for each axis. Patterning across the DV axis requires interaction between the Toll and Dpp/TGF-β pathways, whereas patterning across the AP axis requires gradients of bicoid/orthodenticle proteins and the actions of a hierarchy of gene transcription factors. We examined the expression and function of Toll and Dpp signalling during honeybee embryogenesis to assess to the role of these genes in DV patterning.

Results

Pathway components that are required for dorsal specification in Drosophila are expressed in an AP-restricted pattern in the honeybee embryo, including Dpp and its receptor Tkv. Components of the Toll pathway are expressed in a more conserved pattern along the ventral axis of the embryo. Late-stage embryos from RNA interference (RNAi) knockdown of Toll and Dpp pathways had both DV and AP patterning defects, confirmed by staining with Am-sna, Am-zen, Am-eve, and Am-twi at earlier stages. We also identified two orthologues of dorsal in the honeybee genome, with one being expressed during embryogenesis and having a minor role in axis patterning, as determined by RNAi and the other expressed during oogenesis.

Conclusions

We found that early acting pathways (Toll and Dpp) are involved not only in DV patterning but also AP patterning in honeybee embryogenesis. Changes to the expression patterns and function of these genes may reflect evolutionary changes in the placement of the extra-embryonic membranes during embryogenesis with respect to the AP and DV axes.

【 授权许可】

   
2014 Wilson et al.; licensee BioMed Central Ltd.

【 图 表 】

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