Renal denervation decreases effective refractory period but not inducibility of ventricular fibrillation in a healthy porcine biomodel: a case control study

Journal of Translational Medicine

Otomar Kittnar²  Ales Linhart⁴  Petr Neuzil¹  Miroslav Chochola⁴  Mikulas Mlcek²  Jaroslav Kudlicka³  Jean-Claude Lubanda⁴ 

[1] Department of Cardiology, Na Homolce Hospital, Roentgenova 2/37, Prague 5 150 30, Czech Republic;Institute of Physiology, First Faculty of Medicine, Charles University in Prague, Albertov 5, Prague 2 128 00, Czech Republic;3rd Department of Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital, U Nemocnice 2, Prague 2 128 00, Czech Republic;2nd Department of Medicine - Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, Prague 2 128 00, Czech Republic

关键词: Biomodel;    Electrophysiological study;    Ventricular fibrillation;    Renal denervation;   

Others  :  1146394
DOI  :  10.1186/s12967-014-0367-y

 received in 2014-11-01, accepted in 2014-12-16,  发布年份 2015

【 摘 要 】

Background

Ventricular arrhythmias play an important role in cardiovascular mortality especially in patients with impaired cardiac and autonomic function. The aim of this experimental study was to determine, if renal denervation (RDN) could decrease the inducibility of ventricular fibrillation (VF) in a healthy porcine biomodel.

Methods

Controlled electrophysiological study was performed in 6 biomodels 40 days after RDN (RDN group) and in 6 healthy animals (control group). The inducibility of VF was tested by programmed ventricular stimulation from the apex of right ventricle (8 basal stimuli coupled with up to 4 extrastimuli) always three times in each biomodel using peripheral extracorporeal oxygenation for hemodynamic support. Further, basal heart rate (HR), PQ and QT intervals and effective refractory period of ventricles (ERP) were measured. Technical success of RDN was evaluated by histological examination.

Results

According to histological findings, RDN procedure was successfully performed in all biomodels. Comparing the groups, basal HR was lower in RDN group: 79 (IQR 58; 88) vs. 93 (72; 95) beats per minute (p = 0.003); PQ interval was longer in RDN group: 145 (133; 153) vs. 115 (113; 120) ms (p < 0.0001) and QTc intervals were comparable: 402 (382; 422) ms in RDN vs. 386 (356; 437) ms in control group (p = 0.1). ERP was prolonged significantly in RDN group: 159 (150; 169) vs. 140 (133; 150) ms (p = 0.001), but VF inducibility was the same (18/18 vs. 18/18 attempts).

Conclusions

RDN decreased the influence of sympathetic nerve system on the heart conduction system in healthy porcine biomodel. However, the electrophysiological study was not associated with a decrease of VF inducibility after RDN.

【 授权许可】

   
2015 Lubanda et al.; licensee BioMed Central.

【 图 表 】

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