Who let the dogs out?: detrimental role of Galectin-3 in hypoperfusion-induced retinal degeneration

Journal of Neuroinflammation

Linnéa Taylor¹  Tomas Deierborg²  Karin Arnér¹  Oscar Manouchehrian¹ 

[1] Department of Ophthalmology, BMC, Lund University, Klinikgatan 26, Lund S-22184, Sweden;Experimental Neuroinflammation Laboratory, BMC, Lund University, Klinikgatan 26, Lund S-22184, Sweden

关键词: Ischemia;    Gliosis;    Neuroinflammation;    Müller cells;    Galectin 3;    Microglia;    Retina;   

Others  :  1222004
DOI  :  10.1186/s12974-015-0312-x

 received in 2014-11-25, accepted in 2015-04-28,  发布年份 2015

【 摘 要 】

Background

Retinal ischemia results in a progressive degeneration of neurons and a pathological activation of glial cells, resulting in vision loss. In the brain, progressive damage after ischemic insult has been correlated to neuroinflammatory processes involving microglia. Galectin-3 has been shown to mediate microglial responses to ischemic injury in the brain. Therefore, we wanted to explore the contribution of Galectin-3 (Gal-3) to hypoperfusion-induced retinal degeneration in mice.

Methods

Gal-3 knockout (Gal-3 KO) and wildtype (WT) C57BL/6 mice were subjected to chronic cerebral hypoperfusion by bilateral narrowing of the common carotid arteries using metal coils resulting in a 30% reduction of blood flow. Sham operated mice served as controls. After 17 weeks, the mice were sacrificed and the eyes were analyzed for retinal architecture, neuronal cell survival, and glial reactivity using morphological staining and immunohistochemistry.

Results

Hypoperfusion caused a strong increase in Gal-3 expression and microglial activation in WT mice, coupled with severe degenerative damage to all retinal neuronal subtypes, remodeling of the retinal lamination and Müller cell gliosis. In contrast, hypoperfused Gal-3 KO mice displayed a retained laminar architecture, a significant preservation of photoreceptors and ganglion cell neurons, and an attenuation of microglial and Müller cell activation.

Conclusion

Moderate cerebral blood flow reduction in the mouse results in severe retinal degenerative damage. In mice lacking Gal-3 expression, pathological changes are significantly attenuated. Gal-3 is thereby a potential target for treatment and prevention of hypoperfusion-induced retinal degeneration and a strong candidate for further research as a factor behind retinal degenerative disease.

【 授权许可】

   
2015 Manouchehrian et al.; licensee BioMed Central.

【 图 表 】

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