Leukocyte infiltration across the blood-spinal cord barrier is modulated by sleep fragmentation in mice with experimental autoimmune encephalomyelitis

Fluids and Barriers of the CNS

Weihong Pan²  Jidong Fang⁴  Pramod K Mishra³  Abba J Kastin¹  Yi He¹  Hung Hsuchou¹  Junyun He¹ 

[1] Blood-Brain Barrier Group, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA;BioPotentials Sleep Center, 8032 Summa Ave, Ste A, Baton Rouge, LA 70809, USA;Department of Biology, University of Texas, San Antonio, TX 78249, USA;Department of Psychiatry, Pennsylvania State University, Hershey, PA 17033, USA

关键词: Spinal cord;    Sleep fragmentation;    Circadian rhythm;    Leukocytes;    EAE;   

Others  :  1098226
DOI  :  10.1186/2045-8118-11-27

 received in 2014-10-17, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

We have recently shown that mice with experimental autoimmune encephalomyelitis (EAE) have increased sleep fragmentation (SF) and reduced sleep efficiency, and that the extent of SF correlates with the severity of disease. It is not yet clear whether and how sleep promotes recovery from autoimmune attacks. We hypothesized that SF promotes leukocyte infiltration across the blood-spinal cord barrier, impairs immune regulation, and thus worsens EAE.

Methods

Three groups of C57 mice were studied: Resting EAE; SF EAE with the mice subjected to the SF maneuver 12 h /day during zeitgeber time (ZT) 0–12 h; and naïve controls with neither EAE nor SF. Besides monitoring of the incidence and severity of EAE, the immune profiles of leukocytes in the spinal cord as well as those in the spleen were determined.

Results

When analyzed 16 days after EAE induction, at which time the SF was terminated, the SF group had a greater number of CD4⁺ T cells and a higher percent of CD4⁺ cells among all leukocytes in the spinal cord than the resting EAE group. When allowed to recover to 28 days after EAE induction, the SF mice had lower EAE scores than the resting EAE group. EAE induced splenomegaly and an increase of Gr1⁺CD11b⁺ myeloid-derived suppressor cells in the splenocytes. However, SF treatment had no additional effect on either peripheral splenocytes or granulocytes that reached the spinal cord.

Conclusion

The SF maneuver facilitated the migration of encephalopathic lymphocytes into the spinal cord. Paradoxically, these mice had a better EAE score after cessation of SF compared with mice without SF.

【 授权许可】

   
2014 He et al.; licensee BioMed Central.

【 图 表 】

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