Positron emission tomography detection of human endothelial cell and fibroblast monolayers: effect of pretreament and cell density on 18FDG uptake

Journal of Angiogenesis Research

Roger Lecomte²  Patrick Vermette¹  Jean-François Beaudoin²  Jacques A Rousseau²  Julie A Chouinard¹ 

[1] Research Centre on Aging, Université de Sherbrooke, 1036, rue Belvédère Sud, Sherbrooke, Québec J1H 4C4, Canada;Sherbrooke Molecular Imaging Centre, Department of Nuclear Medicine and Radiobiology, Université de Sherbrooke, 3001, 12ième Avenue Nord, Sherbrooke, Québec J1H 5N4, Canada

关键词: Tissue Engineering;    Fibroblasts;    Endothelial Cells;    HUVEC;    Positron Emission Tomography (PET);    FDG;   

Others  :  802045
DOI  :  10.1186/2045-824X-4-5

 received in 2012-01-11, accepted in 2012-03-20,  发布年份 2012

【 摘 要 】

Background

The non-destructive assessment and characterization of tridimensional (3D) cell and tissue constructs in bioreactors represents a challenge in tissue engineering. Medical imaging modalities, which can provide information on the structure and function of internal organs and tissues in living organisms, have the potential of allowing repetitive monitoring of these 3D cultures in vitro. Positron emission tomography (PET) is the most sensitive non-invasive imaging modality, capable of measuring picomolar amounts of radiolabeled molecules. However, since PET imaging protocols have been designed almost exclusively for in vivo investigations, suitable methods must be devised to enable imaging of cells or tissue substitutes. As a prior step to imaging 3D cultures, cell radiotracer uptake conditions must first be optimized.

Methods

In this study, human umbilical vein endothelial cells (HUVEC) and human fibroblasts were cultured at different densities and PET was used to non-destructively monitor their glycolytic activity by measuring ¹⁸F-fluorodeoxyglucose (¹⁸FDG) uptake. Various cell preconditioning protocols were investigated by adjusting the following parameters to optimize ¹⁸FDG uptake: glucose starvation, insulin stimulation, glucose concentration, ¹⁸FDG incubation time, cell density and radiotracer efflux prevention.

Results

The conditions yielding optimal ¹⁸FDG uptake, and hence best detection sensitivity by PET, were as follows: 2-hour cell preconditioning by glucose deprivation with 1-hour insulin stimulation, followed by 1-hour ¹⁸FDG incubation and 15-minute stabilization in standard culture medium, prior to rinsing and PET scanning.

Conclusions

A step-wise dependence of ¹⁸FDG uptake on glucose concentration was found, but a linear correlation between PET signal and cell density was observed. Detection thresholds of 36 ± 7 and 21 ± 4 cells were estimated for endothelial cells and fibroblasts, respectively.

【 授权许可】

   
2012 Chouinard et al; licensee BioMed Central Ltd.

【 图 表 】

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