Melittin restores proteasome function in an animal model of ALS

Journal of Neuroinflammation

Sun-Mi Choi²  Sang Min Lee²  Sun Choel Yang¹  Seon Hwy Kim²  Eun Jin Yang² 

[1] Department of Instrument Development, Korea Basic Science Institute, 113, Gwahag-ro, Yuseong-gu, Daejeon, 305-333, Korea;Department of Standard Research, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejeon, 305-811, Korea

关键词: α-synuclein;    Amyotrophic lateral sclerosis (ALS);    Melittin;    hSOD1^G93A;   

Others  :  1213299
DOI  :  10.1186/1742-2094-8-69

 received in 2011-05-12, accepted in 2011-06-20,  发布年份 2011

【 摘 要 】

Amyotrophic lateral sclerosis (ALS) is a paralyzing disorder characterized by the progressive degeneration and death of motor neurons and occurs both as a sporadic and familial disease. Mutant SOD1 (mtSOD1) in motor neurons induces vulnerability to the disease through protein misfolding, mitochondrial dysfunction, oxidative damage, cytoskeletal abnormalities, defective axonal transport- and growth factor signaling, excitotoxicity, and neuro-inflammation.

Melittin is a 26 amino acid protein and is one of the components of bee venom which is used in traditional Chinese medicine to inhibit of cancer cell proliferation and is known to have anti-inflammatory and anti-arthritic effects.

The purpose of the present study was to determine if melittin could suppress motor neuron loss and protein misfolding in the hSOD1^G93A mouse, which is commonly used as a model for inherited ALS. Meltittin was injected at the 'ZuSanLi' (ST36) acupuncture point in the hSOD1^G93A animal model. Melittin-treated animals showed a decrease in the number of microglia and in the expression level of phospho-p38 in the spinal cord and brainstem. Interestingly, melittin treatment in symptomatic ALS animals improved motor function and reduced the level of neuron death in the spinal cord when compared to the control group. Furthermore, we found increased of α-synuclein modifications, such as phosphorylation or nitration, in both the brainstem and spinal cord in hSOD1^G93A mice. However, melittin treatment reduced α-synuclein misfolding and restored the proteasomal activity in the brainstem and spinal cord of symptomatic hSOD1^G93A transgenic mice.

Our research suggests a potential functional link between melittin and the inhibition of neuroinflammation in an ALS animal model.

【 授权许可】

   
2011 Yang et al; licensee BioMed Central Ltd.

【 图 表 】

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