Dihydrotestosterone induces SREBP-1 expression and lipogenesis through the phosphoinositide 3-kinase/Akt pathway in HaCaT cells

Lipids in Health and Disease

Dan Luo¹  Zhi-qiang Yin¹  Di Wu¹  Yang Xu¹  Qiu-hong Huang¹  Bing-rong Zhou¹ 

[1] Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China

关键词: HaCaT cells;    Phosphoinositide 3-kinase/Akt;    SREBP-1;    Dihydrotestosterone;   

Others  :  1160144
DOI  :  10.1186/1476-511X-11-156

 received in 2012-09-08, accepted in 2012-10-27,  发布年份 2012

【 摘 要 】

Background

The purpose of this study was to investigate the effects and mechanisms of dihydrotestosterone (DHT)-induced expression of sterol regulatory element binding protein-1 (SREBP-1), and the synthesis and secretion of lipids, in HaCaT cells. HaCaT cells were treated with DHT and either the phosphoinositide 3-kinase inhibitor LY294002 or the extracellular-signal-regulated kinase (ERK) inhibitor PD98059. Real time-PCR, Western blot, Oil Red staining and flow cytometry were employed to examine the mRNA and protein expressions of SREBP-1, the gene transcription of lipid synthesis, and lipid secretion in HaCaT cells.

Findings

We found that DHT upregulated mRNA and protein expressions of SREBP-1. DHT also significantly upregulated the transcription of lipid synthesis-related genes and increased lipid secretion, which can be inhibited by the addition of LY294002.

Conclusions

Collectively, these results indicate that DHT induces SREBP-1 expression and lipogenesis in HaCaT cells via activation of the phosphoinositide 3-kinase/Akt Pathway.

【 授权许可】

   
2012 Zhou et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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