Aging is associated with highly defined epigenetic changes in the human epidermis

Epigenetics & Chromatin

Frank Lyko⁴  Marc Winnefeld¹  Asaf Hellman²  Lars Kaderali³  Pranav P Kulkarni³  Jörn Söhle¹  Dvir Aran²  Sabine Hagemann¹  Günter Raddatz⁴ 

[1] Research & Development, Beiersdorf AG, Unnastrasse 48, Hamburg 20253, Germany;Institute for Medical Research Israel-Canada, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel;Institute for Medical Informatics and Biometry, Dresden University of Technology, Fetscherstrasse 74, Dresden 01307, Germany;Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Im Neuenheimer Feld 580, Heidelberg 69120, Germany

关键词: Transcriptome sequencing;    Methylome sequencing;    Epidermis;    DNA methylation;    Aging;   

Others  :  805263
DOI  :  10.1186/1756-8935-6-36

 received in 2013-09-25, accepted in 2013-10-08,  发布年份 2013

【 摘 要 】

Background

Altered DNA methylation patterns represent an attractive mechanism for understanding the phenotypic changes associated with human aging. Several studies have described global and complex age-related methylation changes, but their structural and functional significance has remained largely unclear.

Results

We have used transcriptome sequencing to characterize age-related gene expression changes in the human epidermis. The results revealed a significant set of 75 differentially expressed genes with a strong functional relationship to skin homeostasis. We then used whole-genome bisulfite sequencing to identify age-related methylation changes at single-base resolution. Data analysis revealed no global aberrations, but rather highly localized methylation changes, particularly in promoter and enhancer regions that were associated with altered transcriptional activity.

Conclusions

Our results suggest that the core developmental program of human skin is stably maintained through the aging process and that aging is associated with a limited destabilization of the epigenome at gene regulatory elements.

【 授权许可】

   
2013 Raddatz et al.; licensee BioMed Central Ltd.

【 图 表 】

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