Serum concentrations of haptoglobin and haptoglobin-matrix metalloproteinase 9 (Hp-MMP 9) complexes of bovine calves in a bacterial respiratory challenge model

BMC Veterinary Research

Jeffrey Lakritz²  Paul J Plummer⁵  Chong Wang³  Vickie L Cooper¹  Renee D Dewell⁴  Grant A Dewell¹  Christy J Hanthorn¹ 

[1] Department of Veterinary and Diagnostic Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, IA, USA;Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus 43210, OH, USA;Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames 50011, IA, USA;Department of Veterinary Microbiology and Preventive Medicine, Center for Food Security and Public Health, College of Veterinary Medicine, Iowa State University, Ames 50011, IA, USA;Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, IA, USA

关键词: Calf;    Pneumonia;    Bovine Respiratory Disease;    MMP 9;    Haptoglobin;    Hp-MMP 9;   

Others  :  1091962
DOI  :  10.1186/s12917-014-0285-5

 received in 2014-07-11, accepted in 2014-11-19,  发布年份 2014

【 摘 要 】

Background

Serum haptoglobin (Hp) and haptoglobin matrix metalloproteinase 9 complexes (Hp-MMP 9) have been identified as biomarkers with diagnostic potential in cattle with conditions resulting in an acute inflammatory response. The purpose of this study was to evaluate potential diagnostic applications of serum Hp and Hp-MMP 9 concentrations in calves with BRD and establish a timeline for their detection in calves experimentally challenged with Bibersteinia trehalosi and Mannheimia haemolytica. Thirty-five cross bred dairy calves were inoculated via tracheal catheterization with either a PCR confirmed leukotoxin negative B. trehalosi isolate, a PCR confirmed leukotoxin positive B. trehalosi isolate, a Mannheimia haemolytica isolate, a combination of leukotoxin negative B. trehalosi and M. haemolytica, or a negative control. Serum samples were collected throughout the study. Calves were euthanized and necropsy performed on day 10 post inoculation.

Results

M. haemolytica inoculated calves had increased lung involvement. Serum Hp and Hp- MMP 9 concentrations were elevated compared to the other treatment groups. Increases in serum Hp and Hp-MMP 9 concentrations for the M. haemolytica group were significantly different from other study groups on day 7 of the study. B. trehalosi inoculated calves did not have increased lung involvement compared to control calves, but the leukotoxin positive B. trehalosi group demonstrated increased serum Hp-MMP 9 concentrations from day 3 to the end of the study compared to the pre-inoculation concentrations.

Conclusion

Serum Hp-MMP 9 concentration is a useful diagnostic tool for detecting early pulmonary inflammation in calves challenged with B. trehalosi and M. haemolytica. Serum Hp-MMP 9 may also be a useful tool in detecting subclinical pulmonary inflammation in challenged calves.

【 授权许可】

   
2014 Hanthorn et al.; licensee BioMed Central Ltd.

【 图 表 】

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