Flaxseed oil increases aortic reactivity to phenylephrine through reactive oxygen species and the cyclooxygenase-2 pathway in rats

Lipids in Health and Disease

Alessandra S Padilha¹  Dalton V Vassallo²  Ivanita Stefanon¹  Marito Afonso Sousa Costa Silva¹  Gilson Brás Broseghini-Filho¹  Camila Cruz Pereira Almenara¹  Dieli Oliveira Nunes¹ 

[1] Department of Physiological Sciences, Federal University of Espirito Santo, Av. Marechal Campos, 1468, Maruípe, 29040-091 Vitória, ES, Brazil;Health Science Center of Vitoria - EMESCAM, Vitoria, Espirito Santo, Brazil

关键词: Oxidative stress;    Vascular reactivity;    Aorta;    Flaxseed oil;    Omega-3;   

Others  :  821588
DOI  :  10.1186/1476-511X-13-107

 received in 2014-04-17, accepted in 2014-06-23,  发布年份 2014

【 摘 要 】

Background

Flaxseed oil has the highest concentration of omega-3 α-linolenic acid, which has been associated with cardiovascular benefit. However, the mechanism underlying the vascular effects induced through flaxseed oil is not well known. Thus, in the present study, we investigated the effects of flaxseed oil on vascular function in isolated rat aortic rings.

Methods

Wistar rats were treated daily with flaxseed oil or a control (mineral oil) intramuscular (i.m.) for fifteen days. Isolated aortic segments were used to evaluate cyclooxygenase-2 (COX-2) protein expression, superoxide anion levels and vascular reactivity experiments.

Results

Flaxseed oil treatment increased the vasoconstrictor response of aortic rings to phenylephrine. Endothelium removal increased the response to phenylephrine in aortic segments isolated from both groups, but the effect was smaller in the treated group. L-NAME incubation similarly increased the phenylephrine response in segments from both groups. The TXA₂ synthase inhibitor furegrelate, the selective COX-2 inhibitor NS 398, the TP receptor antagonist SQ 29.548, the reactive oxygen species (ROS) scavenger apocynin, the superoxide anion scavengers tiron and the phospholipase A₂ inhibitor dexamethasone partially reversed the flaxseed oil-induced increase in reactivity to phenylephrine.

Conclusions

These findings suggest that flaxseed oil treatment increased vascular reactivity to phenylephrine through an increase in ROS production and COX-2-derived TXA₂ production. The results obtained in the present study provide new insight into the effects of flaxseed oil treatment (i.m.) on vascular function.

【 授权许可】

   
2014 Nunes et al.; licensee BioMed Central Ltd.

【 图 表 】

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