Flaxseed oil increases aortic reactivity to phenylephrine through reactive oxygen species and the cyclooxygenase-2 pathway in rats

Lipids in Health and Disease

Research

Gilson Brás Broseghini-Filho¹  Ivanita Stefanon¹  Alessandra S Padilha¹  Camila Cruz Pereira Almenara¹  Marito Afonso Sousa Costa Silva¹  Dieli Oliveira Nunes¹  Dalton V Vassallo² 

[1] Department of Physiological Sciences, Federal University of Espirito Santo, Av. Marechal Campos, 1468, Maruípe, 29040-091, Vitória, ES, Brazil;Department of Physiological Sciences, Federal University of Espirito Santo, Av. Marechal Campos, 1468, Maruípe, 29040-091, Vitória, ES, Brazil;Health Science Center of Vitoria - EMESCAM, Vitoria, Espirito Santo, Brazil;

关键词: Omega-3;    Flaxseed oil;    Aorta;    Vascular reactivity;    Oxidative stress;   

DOI  :  10.1186/1476-511X-13-107

 received in 2014-04-17, accepted in 2014-06-23,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundFlaxseed oil has the highest concentration of omega-3 α-linolenic acid, which has been associated with cardiovascular benefit. However, the mechanism underlying the vascular effects induced through flaxseed oil is not well known. Thus, in the present study, we investigated the effects of flaxseed oil on vascular function in isolated rat aortic rings.MethodsWistar rats were treated daily with flaxseed oil or a control (mineral oil) intramuscular (i.m.) for fifteen days. Isolated aortic segments were used to evaluate cyclooxygenase-2 (COX-2) protein expression, superoxide anion levels and vascular reactivity experiments.ResultsFlaxseed oil treatment increased the vasoconstrictor response of aortic rings to phenylephrine. Endothelium removal increased the response to phenylephrine in aortic segments isolated from both groups, but the effect was smaller in the treated group. L-NAME incubation similarly increased the phenylephrine response in segments from both groups. The TXA2 synthase inhibitor furegrelate, the selective COX-2 inhibitor NS 398, the TP receptor antagonist SQ 29.548, the reactive oxygen species (ROS) scavenger apocynin, the superoxide anion scavengers tiron and the phospholipase A2 inhibitor dexamethasone partially reversed the flaxseed oil-induced increase in reactivity to phenylephrine.ConclusionsThese findings suggest that flaxseed oil treatment increased vascular reactivity to phenylephrine through an increase in ROS production and COX-2-derived TXA2 production. The results obtained in the present study provide new insight into the effects of flaxseed oil treatment (i.m.) on vascular function.

【 授权许可】

Unknown   
© Nunes et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.