The protective effect of Malva sylvestris on rat kidney damaged by vanadium

Lipids in Health and Disease

Abdelfattah El Feki³  Sofiane Bezzine²  Jean-Claude Murat¹  Ahlem Soussi³  Wafa Marouane² 

[1] Laboratoire de Biologie Cellulaire, Faculté de Médecine, Toulouse, France;Laboratoire de Biochimie, Institut Supérieur de Biotechnologie de Sfax, Route de Soukra 3038 Sfax - University of Sfax-Tunisia;Laboratoire d'Ecophysiologie Animale, Faculté des Sciences, Route de Soukra 3038 Sfax - University of Sfax-Tunisia

关键词: antioxidant enzymes;    oxidative stress;    nephrotoxicity;    Malva sylvestris;    Ammonium metavanadate;   

Others  :  1212673
DOI  :  10.1186/1476-511X-10-65

 received in 2011-02-04, accepted in 2011-04-23,  发布年份 2011

【 摘 要 】

Background

The protective effect of the common mallow (Malva sylvestris) decoction on renal damages in rats induced by ammonium metavanadate poisoning was evaluated. On the one hand, vanadium toxicity is associated to the production of reactive oxygen species, causing a lipid peroxidation and an alteration in the enzymatic antioxidant defence. On the other hand, many medicinal plants are known to possess antioxidant and radical scavenging properties, thanks to the presence of flavonoids. These properties were confirmed in Malva sylvestris by two separate methods; namely, the Diphenyl-2-picrylhydrazyl assay and the Nitroblue Tetrazolium reduction assay.

Results

In 80 rats exposed to ammonium metavanadate (0.24 mmol/kg body weight in drinking water) for 90 days, lipid peroxidation levels and superoxide dismutase, catalase and glutathione peroxidase activities were measured in kidney. A significant increase in the formation of free radicals and antioxidant enzyme activities was noticed. In addition, a histological examination of kidney revealed a structural deterioration of the renal cortical capsules and a shrinking of the Bowman space. In animals intoxicated by metavanadate but also given a Malva sylvestris decoction (0.2 g dry mallow/kg body weight), no such pathologic features were observed: lipid peroxidation levels, antioxidant enzyme activities and histological features appeared normal as compared to control rats.

Conclusion

Malva sylvestris is proved to have a high antioxidative potential thanks to its richness in phenolic compounds.

【 授权许可】

   
2011 Marouane et al; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Barceloux DG: Vanadium. Clin Toxicol 1999, 37:265-278.
• [2]WHO Regional Office for Europe: Vanadium. Air Quality Guidelines-Second Edition 2000, 12:1-9. Chapter 6;
• [3]Domingo JL, Gomez M, Liobet JM, Corbella J, Keen CL: Prevention by sodium 4,5-dihydroxybenzene1,3-disulfonate (tiron) of vanadium-induced behavioral toxicity in rats. Biol Trace Elem Res 1991, 69:249-259.
• [4]Balasubramanian R, Kasiappan R, Vengidusamy N, Muthusamy K, Sorimuthu S: Protective effect of macrocyclic binuclear oxovanadium complex on oxidative stress in pancreas of streptozotocin induced diabetic rats. Chem Biol Interact 2004, 149:9-21.
• [5]Crans DC: Interaction of vanadates with biogenic ligands. Met Ions Biol Syst 1995, 31:147-209.
• [6]Aureliano M, Madeira VMC: Energy transduction mechanisms as affected by vanadium(V) species: Ca2+-pumping in sarcoplasmic reticulum. In Vanadium in the environment, Part 1: Chemistry and Biochemistry. Edited by Nriagu JO. John Wiley & Sons Inc. New York; 1998:333-357.
• [7]Byczkowski JZ, Kulkarni AP: Oxidative stress and pro-oxidant biological effects of vanadium. In Vanadium in the Environment, Part 2: Health Effects. Volume 31. Edited by Nriagu JO. New York: John Wiley & Sons; 1998::235-264.
• [8]Ishige K, Schubert D, Sagara Y: Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Radic Biol Med 2001, 4:433-446.
• [9]Bors W, Heller W, Michel C, Saran M: Flavonoids as antioxidants: determination of radical-scavenging efficiencies. Method Enzymol 1990, 186:343-55.
• [10]Briviba K, Sies H: Natural antioxidants in Human Health and Disease. In Academic Press. Edited by B. Frei. San Diego, CA; 1994:107-128.
• [11]Bors W, Michel C, Stettmaier K: Antioxidant effects of flavonoïdes. Biofactors 1997, 6:399-402.
• [12]Pietta P: Flavonoids in medicinal plants. In Flavonoids in health and disease. Edited by Rice-Evans CA, Packer L. New York: Marcel Dekker; 1998:61-110.
• [13]Paufique JJ: Method for extracting an active principle based on Malva sylvestris, the active principle obtained, and cosmetic treatment using it. Patent Application 2000, 2000-11973. FR 20000920
• [14]Farina A, Doldo A, Cotichini V, Rajevic M, Quaglia MG, Mulinacci N, Vincieri FF: HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC. Pharmaceut Sci 1995, 14:203-211.
• [15]Barros L, Carvalho AM, Ferreira ICFR: Leaves, flowers, immature fruits and leafy flowered stems of Malva sylvestris: A comparative study of the nutraceutical potential and composition. Food Chem Toxicol 2010, 48:1466-1472.
• [16]Nawwar MAM, Buddrus J: A gossypetin glucuronide sulphate from the leaves of Malva sylvestris. Phytochemstry 1981, 20:2446-2448.
• [17]Billeter M, Meier B, Sticher O: 8-Hydroxyflavonoid Glucuronides from Malva sylvestris. Phytochemistry 1991, 3:987-990.
• [18]De La Torre A, Granero S, Mayayo E, Corbella J, Domingo JL: Effect of age on vanadium nephrotoxicity in rats. Toxicol Lett 1999, 05:75-82.
• [19]Yagi K: A simple Fluorometric assay for lipoperoxide in blood plasma. Biochem Med 1976, 212-216.
• [20]Asada K, Takahashi M, Nagate M: Assay and inhibitors of spinach superoxide dismutase. Agr Biol Chem 1974, 471-473.
• [21]Aebi H: Catalase. In Methods of Enzymatic Analysis. Edited by HU Bergmeyer. Academ Press; 1974:673-684.
• [22]Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidise. Lab Clin Med 1967, 158-169.
• [23]Gulcin I, Berashvili D, Gepdiremen A: Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne. Ethnopharmacology 2005, 101:287-293.
• [24]DellaGreca M, Cutillo F, D'Abrosca B, Fiorentino A, Pacifico S, Zarrelli A: Antioxidant and radical scavenging properties of Malva sylvestris. Natl Prod 2009, 4:893-896.
• [25]El SN, Karakaya S: Radical scavenging and iron-chelating activities of some greens used as traditional dishes in Mediterranean diet. Food Sci Nutr 2004, 55:67-74.
• [26]Annan K, Houghton PJ: Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana. Ethnopharmacology 2008, 119:141-144.
• [27]Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Park SH, Kim SK: Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci 2002, 163:1161-1168.
• [28]Yazdanparast R, Bahramikia S, Ardestani A: Nasturtium officinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact 2008, 172:176-184.
• [29]Shi H, Hudson LG, Liu KJ: Oxidative stress and apoptosis in metal ion-induced carcinogenesis. Free Rad Biol Med 2004, 37:582-593.
• [30]Schaich KM: Metals and lipid oxidation. Contemp issues Lip 1992, 27:209-218.
• [31]Scibior A, Zaporowska H: Lipid peroxidation in liver and kidneys of rats treated with vanadium and/or chromium. In Proceedings of the Sixth Winter Research Conference on Free radicals in Enzymatic and Biomimetic Catalysis. Les Houches France; 2003:23-24.
• [32]Scibior A, Zaporowska H, Ostrowski J, Banach A: Combined effect of vanadium(V) and chromium(III) on lipid peroxidation in liver and kidney of rats. Chem Biol Interact 2006, 159:213-222.
• [33]Soussi A, Croute F, Soleilhavoup JP, Kammoun A, El Feki A: Impact of green tea on oxidative stress induced by ammonium metavanadate exposure in male rats. Cr Biol 2006, 329:775-784.
• [34]Elingold I, Isollabella MP, Casanova MB, Celentano AM, P'erez C, Cabrera JL, Diez RA, Dubin M: Mitochondrial toxicity and antioxidant activity of a prenylated flavonoid isolated from Dalea elegans. Chem Biol Interact 2008, 171:294-305.
• [35]Shaw S, Rubin K, Lieber CS: Depressed hepatic glutathione and increased diene conjugates in alcoholic liver disease: evidence of lipid peroxidation. Dig Dis Sci 1993, 585-589.
• [36]Aureliano M, Joaquim N, Sousa A, Martins H, Coucelo JM: Oxidative stress in toadfish (Halobactrachus didactylus) cardiac muscle, Acute exposure to vanadate oligomers. Inorg Biochem 2002, 90:159-165.
• [37]Yanardag R, Bolkent S, Karabulut-Bulan O, Tunali S: Effects of vanadyl sulfate on kidney in experimental diabetes. Biol Trace Elem Res 2003, 95:73-85.
• [38]Ramachandran B, Ravi K, Narayanan V, Kandaswamy M, Subramanian S: Effect of macrocyclic binuclear oxovanadium complex on tissue defense system in streptozotocin-induced diabetic rats. Clin Chem 2004, 345:141-150.
• [39]S'cibior A, Zaporowska H: Effects of combined vanadate and magnesium treatment on erythrocyte antioxidant defence system in rats. Env Toxicol Pharmacol 2010, 30:153-161.
• [40]Soares SS, Martins H, Duarte RO, Moura JJG, Coucelo J, Gutie'rrez-Merino C, Aureliano M: Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration. Inorg Biochem 2007, 101:80-88.
• [41]Oboh G, Rocha JBT: Antioxidant and neuroprotective properties of sour tea (Hibiscus sabdariffa, calyx) and green tea (Camellia sinensis) on some prooxidant- induced lipid peroxidation in brain in vitro. Food Biophys 2008, 3:382-389.
• [42]Ilavarasan R, Vasudevan M, Anbazhagan S, Venkataraman S: Antioxidant activity of Thespesia populnea bark extracts against carbon tetrachloride-induced liver injury in rats. Ethnopharmacology 2003, 87:227-230.
• [43]Al-Bayati MA, Xie Y, Mohr FC, Margolin S B, Giri S N: Effect of pirfenidone against vanadate-induced kidney fibrosis in rats. Biochem Pharmacol 2002, 64:517-525.
• [44]Mohamed M, Abdellatif MD, Sabar A, Elglammal MD: Sodium fluoride ion and renal function after prolonged sevoflurane or isoflurane anaesthesia. Eng J Ana 2003, 19:78-83.
• [45]Mohan M, Kamble S, Gadhi P, Kasture S: Protective effect of Solanum torvum on doxorubicin-induced nephrotoxicity in rats. Food and Chem Toxicol 2010, 48:436-440.
• [46]Woottisin S, Hossain RZ, Yachantha C, Sriboonlue P, Ogawa Y, Saito S: Effects of Orthosiphon grandiflorus, Hibiscus sabdariffa and Phyllanthus amarus Extracts on Risk Factors for Urinary Calcium Oxalate Stones in Rats. Urology 2011, 185:323-328.
• [47]Lin YL, Cheng CY, Lin YP, Lau YW, Juan IM, Lin JK: Hypolipidemic effect of green tea leaves through induction of antioxidant and phase II enzymes including superoxide dismutase, catalase, and glutathione S-transferase in rats. Agricl Biol Chem 1998, 46:1893-1899.
• [48]Shahidi F, Wanasundara PKJPD: Phenolic antioxidants. Food Sci Nutr 1992, 32:67-103.
• [49]Sanchez-Moreno C, Larrauri JA, Saura-Calixto F: Free radical scavenging capacity an inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res Inter 1999, 32:407-412.
• [50]Koleva I, Van beek TA, Linssn JP, De groot A, Evstatieva LN: Screening of plant extracts of antioxydant activity: A compare study on three testing methods. Phytochem Anal 2002, 13:8-17.
• [51]Steafan IL, Fridovichi I: Superoxide from glucose oxidase or from Nitoblue Tetrazolium. Biochem and Biophys 1995, 318:408-10.
• [52]Lowry OH, Rosenbroug NJ, Randall R: Protein measurement with the folin phenol reagent. Biol Chem 1951, 265-271.