A rapid field test for the measurement of bovine serum immunoglobulin G using attenuated total reflectance infrared spectroscopy

BMC Veterinary Research

Gregory P. Keefe²  R. Anthony Shaw¹  Christopher B. Riley³  J. Trenton McClure²  Siyuan Hou²  Ibrahim Elsohaby⁴ 

[1]National Research Council of Canada, Medical Devices Portfolio, Winnipeg R3B 1Y6, MB, Canada

[2]Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown PEI C1A 4P3, Canada

[3]Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North 4442, New Zealand

[4]Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia Province, Egypt

关键词: Immunoglobulin G;    Attenuated total reflectance infrared spectroscopy;    Failure of transfer of passive immunity;    Bovine;   

Others  :  1224305
DOI  :  10.1186/s12917-015-0539-x

 received in 2015-02-02, accepted in 2015-08-10,  发布年份 2015

【 摘 要 】

Background

Following the recent development of a new approach to quantitative analysis of IgG concentrations in bovine serum using transmission infrared spectroscopy, the potential to measure IgG levels using technology and a device better designed for field use was investigated. A method using attenuated total reflectance infrared (ATR) spectroscopy in combination with partial least squares (PLS) regression was developed to measure bovine serum IgG concentrations. ATR spectroscopy has a distinct ease-of-use advantage that may open the door to routine point-of-care testing. Serum samples were collected from calves and adult cows, tested by a reference RID method, and ATR spectra acquired. The spectra were linked to the RID-IgG concentrations and then randomly split into two sets: calibration and prediction. The calibration set was used to build a calibration model, while the prediction set was used to assess the predictive performance and accuracy of the final model. The procedure was repeated for various spectral data preprocessing approaches.

Results

For the prediction set, the Pearson’s and concordance correlation coefficients between the IgG measured by RID and predicted by ATR spectroscopy were both 0.93. The Bland Altman plot revealed no obvious systematic bias between the two methods. ATR spectroscopy showed a sensitivity for detection of failure of transfer of passive immunity (FTPI) of 88 %, specificity of 100 % and accuracy of 94 % (with IgG <1000 mg/dL as the FTPI cut-off value).

Conclusion

ATR spectroscopy in combination with multivariate data analysis shows potential as an alternative approach for rapid quantification of IgG concentrations in bovine serum and the diagnosis of FTPI in calves.

【 授权许可】

   
2015 Elsohaby et al.

【 图 表 】

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