Effects of tobacco smoking during pregnancy on oxidative stress in the umbilical cord and mononuclear blood cells of neonates

Journal of Biomedical Science

Thiago de Melo Costa Pereira²  Elisardo Corral Vasquez⁴  Silvana dos Santos Meyrelles⁴  Breno Valentim Nogueira¹  Jean Pierre Louzada Ramos³  Marcella Leite Porto⁴  Ednildes de Almeida Olympio Rua³ 

[1] Laboratory of Morphology, UFES, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil;Federal Institute of Education, Science and Technology (IFES), Vila Velha, ES, Brazil;Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Av. Comissário José Dantas de Melo, n°21, Boa Vista, Vila Velha, 29102-920, ES, Brazil;Laboratory of Translational Physiology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil

关键词: Apoptosis;    Pregnant women;    Oxidative stress;    Cord blood;    Cigarette smoke;   

Others  :  1139202
DOI  :  10.1186/s12929-014-0105-z

 received in 2014-09-24, accepted in 2014-12-05,  发布年份 2014

【 摘 要 】

Background

Although cigarette smoke is known to be a complex mixture of over 4000 substances that can lead to damage through active or passive smoking, its mechanisms and biochemical consequences in pregnancy and neonates are not yet fully understood. Therefore, in the present study, we propose to study the impact of smoking during gestation on the viability of blood mononuclear cells (MNC) from umbilical cords of newborns to assess the degree of oxidative stress and cell viability. After childbirth, the cord blood and the umbilical cord were immediately collected in public hospitals in Greater Vitoria, ES, Brazil. Flow cytometry was used to analyze the cord blood followed by biochemical and histological tests to analyze possible changes in the umbilical cord.

Results

Pregnant smokers had a reduction of MNC viability from the umbilical cord (10%), an increase in the production of reactive oxygen species (ROS) and an increase in cell apoptosis (~2-fold) compared to pregnant non-smokers. In the umbilical cord, it was observed an increase of advanced oxidation protein products - AOPP (~2.5-fold) and a loss of the typical architecture and disposition of endothelial cells from the umbilical artery.

Conclusions

These data suggest that maternal cigarette smoking during pregnancy (even in small amounts) may compromise the viability of MNC cells and damage the umbilical cord structure, possibly by excessive ROS bioavailability.

【 授权许可】

   
2014 Rua et al.; licensee BioMed Central.

【 图 表 】

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