Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

BMC Veterinary Research

Armanda DS Bastos⁴  Livio Heath²  Adesoji A Makinde³  Margaret M Fasina¹  Olusola O Oladipo³  Oyinlola O Olaokun⁵  Folorunso O Fasina⁴ 

[1] Maximum Farms, P. O. Box 23, Vom Post Office, Plateau State, Nigeria;Transboundary Animal Disease Programme, ARC-Onderstepoort Veterinary Institute, Onderstepoort, South Africa;National Veterinary Research Institute, Vom, Plateau State, Nigeria;Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Hatfield, South Africa;Phytomedicine Programme, Department of Paraclinical Science, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa

关键词: Antiviral;    African swine fever virus;    Ancistrocladus uncinatus;   

Others  :  1119518
DOI  :  10.1186/1746-6148-9-120

 received in 2012-10-30, accepted in 2013-06-10,  发布年份 2013

【 摘 要 】

Background

African swine fever (ASF), a highly contagious fatal acute haemorrhagic viral disease of pigs currently has no treatment or vaccination protocol and it threatens the pig industry worldwide. Recent outbreaks were managed by farmers with ethnoveterinary preparations with various claims of effectiveness.

Results

We identified 35 compounds using GC-MS protocol and ASF virus (NIG 99) was significantly reduced by some extracts and fractions of the plant. However, the plant was poorly extracted by water and cytotoxicity was found to be a major problem with the use of the plant since its extracts also reduced the primary cells used in the assay.

Conclusion

It is confirmed that the plant has antiviral potentials against ASF virus and farmers’ claims seem to have certain degree of veracity, but finding the best means of exploring the potential of the plant while reducing its cytotoxic effect in-vitro and in-vivo will be necessary.

【 授权许可】

   
2013 Fasina et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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