Gender difference following high cholesterol diet induced renal injury and the protective role of rutin and ascorbic acid combination in Wistar albino rats

Lipids in Health and Disease

Abdulaziz S Alroujayee²  Abdulaziz Mohammed Aleisa¹  Osama Abdelrahman Alkhamees³  Hatem Mustafa Abuohashish¹  Salim Salih Al-Rejaie¹ 

[1] Department of Pharmacology and Toxicology, College of Pharmacy, P.O. Box 2457, King Saud University, Riyadh 11451, Saudi Arabia;College of Medicine, Al-Imam University, Riyadh, PO Box 11623, Saudi Arabia;Department of Pharmacology, College of Medicine, Al-Imam University, Riyadh, PO Box 11623, Saudi Arabia

关键词: Ascorbic acid;    Rutin;    Gender difference;    Renal injury;    Nephrotoxicity;    High cholesterol diet;   

Others  :  1160326
DOI  :  10.1186/1476-511X-11-41

 received in 2011-12-26, accepted in 2012-03-16,  发布年份 2012

【 摘 要 】

Background

An increased interest is given to the impact of high fat diet on health worldwide. Abnormalities in lipid metabolism induced by high cholesterol diet (HCD) were reported to exacerbate renal diseases via oxidative stress pathways. Rutin and ascorbic acid showed a protective role against oxidative stress-mediated diseases. Furthermore, both lipid metabolism and tissue response to oxidative stress damage was found to vary according to animal gender. Thus, the objective of this work was to examine possible gender-related differences and the possible protective effects of rutin and ascorbic acid supplementation on high cholesterol diet induced nephrotoxicity.

Methods

96 young male and female Wistar albino rats were used. HCD supplemented animals were treated with rutin alone or in combination with ascorbic acid for 6 weeks. Creatinine plasma level was estimated. Furthermore, kidney levels of nucleic acids, total protein, malondialdehyde (MDA), reduced glutathione (GSH), total cholesterol, and triglycerides were determined. Finally, kidney tissues were used for histopathological examination.

Results

HCD supplementation decreased kidney level of nucleic acids, which was more prominent in female animals. Both vitamin combination significantly attenuated HCD induced decrease in nucleic acids. Moreover, kidney level of MDA was significantly altered by HCD in both genders, which was inhibited by rutin and ascorbic acid alone or in combination in male groups and by both vitamins in female groups. There was a reduction in kidney level of GSH by HCD, especially in male groups, which was attenuated by rutin and ascorbic acid combination. Kidney levels of total cholesterol and triglycerides were significantly increased by HCD supplementation in both genders. Coadministration with rutin and/or ascorbic acid protected from such increase, which was more obvious in both vitamins combination. Histopathological investigation supported vitamins protective effect, which was more prominent in male vitamins combination group.

Conclusions

HCD-induced renal injury in female was higher than in male animals, suggesting a better anti-oxidative stress defense response in male's kidney. Moreover, the antioxidant and reno-protective effects of rutin and ascorbic acid were augmented following their combination.

【 授权许可】

   
2012 Al-Rejaie et al; licensee BioMed Central Ltd.

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