Adenovirus36 infection expresses cellular APMI and Visfatin genes in overweight Uygur individuals

Diagnostic Pathology

Yaqun Guan³  Xian Gong³  Yiliyasi Aisa³  Nuerbiye Nuermaimaiti³  Yanjiao Wang³  Jianfei Lu³  Cheng Zhang²  Kelimu Abudureyimu²  Xi Chang³  Xinmin Mao¹  Yi Jiao³ 

[1] Traditional Chinese Medical College, Xinjiang Medical University, Urumqi 830011, P. R China;Endoscopic Branch, Xinjiang Uygur Autonomous Regional People’s Hospital, Urumqi 830000, P. R China;Department of Biochemistry and Molecular Biology, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830011, P. R China

关键词: Visfatin;    Adiponectin;    Obesity;    Adipocytokines;    Adenovirus 36;   

Others  :  801912
DOI  :  10.1186/1746-1596-9-83

 received in 2014-01-29, accepted in 2014-04-02,  发布年份 2014

【 摘 要 】

Objective

This study is to determine if Adenovirus type 36 (Ad36) infection is related to macrophage infiltration in the obese group and non-obese group and the related molecular mechanisms.

Methods

Ninety obesity patients and 95 non-obesity Uygur individuals were enrolled in this study. CD68 levels in abdominal subcutaneous and omental adipose tissues were detected by immunohistochemistry. The cytokine expression levels of adiponectin (APMI) and visfatin in serum were measured by enzyme-linked immunosorbent assay. Infection of 3T3-L1 cells with Ad36 was performed. Real-time PCR was performed to determine expression levels of APMI and Visfatin genes in the 3T3-L1 preadipocytes infected with Ad36.

Results

In the obese individuals infected with Ad36, the expression levels of adiponectin and visfatin in serum was elevated. For the individuals infected with Ad36, the macrophage infiltration (as indicated by CD68 level) in the obese group was also significantly higher than that in the non-obese group (P < 0.05) in both abdominal subcutaneous and omental adipose tissues. The real-time PCR results indicated that APMI mRNA levels and Visfatin mRNA levels in Ad36 infected cells were significantly increased.

Conclusions

Ad36 infection may be a factor related with macrophage infiltration in adipose tissues of the obese patients. The APMI and Visfatin genes may be involved in the mechanism underlying the effect of Ad36 infection on the obese patients.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1849614638119816 webcite

【 授权许可】

   
2014 Jiao et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Canale MP, Manca Di Villahermosa S, Martino G, Rovella V, Noce A, De Lorenzo A, Di Daniele N: Obesity-Related Metabolic Syndrome: Mechanisms of Sympathetic Overactivity. Int J Endocrinol 2013, 2013:865965.
• [2]Connor JM O, Millar SR, Buckley CM, Kearney PM, Perry IJ: The prevalence and determinants of undiagnosed and diagnosed type 2 diabetes in middle-aged irish adults. PLoS One 2013, 8:e80504.
• [3]Banerjee D, Leong WB, Arora T, Nolen M, Punamiya V, Grunstein R, Taheri S: The Potential Association between Obstructive Sleep Apnea and Diabetic Retinopathy in Severe Obesity-The Role of Hypoxemia. PLoS One 2013, 8:e79521.
• [4]Aung K, Lorenzo C, Hinojosa MA, Haffner SM: Risk of Developing Diabetes and Cardiovascular Disease in Metabolically Unhealthy Normal-Weight and Metabolically Healthy Obese Individuals. J Clin Endocrinol Metab 2013, 20:jc20132832. [Epub ahead of print]
• [5]Díez JJ, Iglesias P: The role of the novel adipocyte-derived hormone adiponectin in human disease. Eur J Endocrinol 2003, 148:293-300.
• [6]Shimomura I: Adipocytokines and life style-related disease. Rinsho Byori 2003, 51:1090-1095.
• [7]Kumada M, Kihara S, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, Arita Y, Okamoto Y, Shimomura I, Hiraoka H, Nakamura T, Funahashi T, Matsuzawa Y: Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol 2003, 23:85-89.
• [8]Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimoto K, Matsuki Y, Murakami M, Ichisaka T, Murakami H, Watanabe E, Takagi T, Akiyoshi M, Ohtsubo T, Kihara S, Yamashita S, Makishima M, Funahashi T, Yamanaka S, Hiramatsu R, Matsuzawa Y, Shimomura I: Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science 2005, 307:426-430.
• [9]Haider DG, Holzer G, Schaller G, Weghuber D, Widhalm K, Wagner O, Kapiotis S, Wolzt M: The adipokine visfatin is markedly elevated in obese children. J Pediatr Gastroenterol Nutr 2006, 43:548-549.
• [10]Zahorska-Markiewicz B, Olszanecka-Glinianowicz M, Janowska J, Kocełak P, Semik-Grabarczyk E, Holecki M, Dabrowski P, Skorupa A: Serum concentration of visfatin in obese women. Metabolism 2007, 56:1131-1134.
• [11]An Y, Yi S, Fitzpatrick A, Gupta V, Prak PR, Oum S, Logerfo JP: Appropriate Body Mass Index and Waist Circumference Cutoff for Overweight and Central Obesity among Adults in Cambodia. PLoS One 2013, 8:e77897.
• [12]Genoni G, Prodam F, Marolda A, Giglione E, Demarchi I, Bellone S, Bona G: Obesity and infection: two sides of one coin. Eur J Pediatr 2014, 173:25-32.
• [13]Pasarica M, Mashtalir N, McAllister EJ, Kilroy GE, Koska J, Permana P, de Courten B, Yu M, Ravussin E, Gimble JM, Dhurandhar NV: Adipogenic human adenovirus Ad-36 induces commitment, differentiation, and lipid accumulation in human adipose-derived stem cells. Stem Cells 2008, 26:969-978.
• [14]Atkinson RL, Dhurandhar NV, Allison DB, Bowen RL, Israel BA, Albu JB, Augustus AS: Human adenovirus-36 is associated with increased body weight and paradoxical reduction of serum lipids. Int J Obes (Lond) 2005, 29:281-286.
• [15]Rogers PM, Fusinski KA, Rathod MA, Loiler SA, Pasarica M, Shaw MK, Kilroy G, Sutton GM, McAllister EJ, Mashtalir N, Gimble JM, Holland TC, Dhurandhar NV: Human adenovirus Ad-36 induces adipogenesis via its E4 orf-1 gene. Int J Obes (Lond) 2008, 32:397-406.
• [16]Lee W: The CEACAM1 expression is decreased in the liver of severely obese patients with or without diabetes. Diagn Pathol 2011, 6:40. BioMed Central Full Text
• [17]Wang L, Cai S, Teng Z, Zhao X, Chen X, Bai X: Insulin therapy contributes to the increased risk of colorectal cancer in diabetes patients: a meta-analysis. Diagn Pathol 2013, 8:180. BioMed Central Full Text
• [18]Nam JH, Na HN, Atkinson RL, Dhurandhar NV, Nam JH, Na HN, Atkinson RL, Dhurandhar NV: Genomic stability of adipogenic human adenovirus 36. Int J Obes (Lond) 2013., 6doi:10.1038/ijo.2013.67 [Epub ahead of print]
• [19]Laing EM, Tripp RA, Pollock NK, Baile CA, Della-Fera MA, Rayalam S, Tompkins SM, Keys DA, Lewis RD: Adenovirus 36, adiposity, and bone strength in late-adolescent females. J Bone Miner Res 2013, 28:489-496.
• [20]Lin WY, Dubuisson O, Rubicz R, Liu N, Allison DB, Curran JE, Comuzzie AG, Blangero J, Leach CT, Göring H, Dhurandhar NV: Long-term changes in adiposity and glycemic control are associated with past adenovirus infection. Diabetes Care 2013, 36:701-707.
• [21]Pasarica M, Loiler S, Dhurandhar NV: Acute effect of infection by adipogenic human adenovirus Ad36. Arch Virol 2008, 153:2097-2102.
• [22]Parra-Rojas I, Del Moral-Hernández O, Salgado-Bernabé AB, Guzmán-Guzmán IP, Salgado-Goytia L, Muñoz-Valle JF: Adenovirus-36 seropositivity and its relation with obesity and metabolic profile in children. Int J Endocrinol 2013, 2013:463194.
• [23]Lumeng CN, Deyoung SM, Bodzin JL, Saltiel AR: Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity. Diabetes 2007, 56:16-23.
• [24]Cani PD, Amar J, Iglesias MA, Poggi M, Knauf C, Bastelica D, Neyrinck AM, Fava F, Tuohy KM, Chabo C, Waget A, Delmée E, Cousin B, Sulpice T, Chamontin B, Ferrières J, Tanti JF, Gibson GR, Casteilla L, Delzenne NM, Alessi MC, Burcelin R: Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 2007, 56:1761-1772.
• [25]Na HN, Nam JH: Adenovirus 36 as an obesity agent maintains the obesity state by increasing MCP-1 and inducing inflammation. J Infect Dis 2012, 205:914-922.
• [26]Kanda H, Tateya S, Tamori Y, Kotani K, Hiasa K, Kitazawa R, Kitazawa S, Miyachi H, Maeda S, Egashira K, Kasuga M: MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Invest 2006, 116:1494-1505.
• [27]Vangipuram SD, Sheele J, Atkinson RL, Holland TC, Dhurandhar NV: A human adenovirus enhances preadipocyte differentiation. Obes Res 2004, 12:770-777.