Effect of engineered TiO2 and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

BMC Veterinary Research

Veronika Kralj-Iglič³  Damjana Drobne⁴  Henry Hägerstrand¹  Deniz Erdogmus⁷  Darko Makovec²  Roman Štukelj³  Jernej Zupanc⁴  Vid Šuštar⁵  Barbara Drašler⁴  Metka Šimundić⁶ 

[1] Department of Biology, Abo Akademi University, Abo/Turku, Finland;J Stefan Institute, Ljubljana, Slovenia;Biomedical Research Group, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia;Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia;Laboratory of Clinical Biophysics, Chair of Orthopaedics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia;Biomedical Research Group, Faculty of Health Sciences, University of Ljubljana and Prva-K Klinika za male živali d.o.o. (Prva-K Clinic for Small Animals), Ljubljana, Slovenia;Department of Electrical and Computer Engineering, Northeastern University, Boston, MA, USA

关键词: Zinc oxide;    Titanium;    Biological membrane;    Phospholipid vesicles;    Dog;    Cancer;    Thrombosis;    Platelet activation;    Erythrocyte shape;    Engineered nanoparticles;   

Others  :  1119631
DOI  :  10.1186/1746-6148-9-7

 received in 2012-09-11, accepted in 2013-01-08,  发布年份 2013

【 摘 要 】

Background

Massive industrial production of engineered nanoparticles poses questions about health risks to living beings. In order to understand the underlying mechanisms, we studied the effects of TiO₂ and ZnO agglomerated engineered nanoparticles (EPs) on erythrocytes, platelet-rich plasma and on suspensions of giant unilamelar phospholipid vesicles.

Results

Washed erythrocytes, platelet-rich plasma and suspensions of giant unilamelar phospholipid vesicles were incubated with samples of EPs. These samples were observed by different microscopic techniques. We found that TiO₂ and ZnO EPs adhered to the membrane of washed human and canine erythrocytes. TiO₂ and ZnO EPs induced coalescence of human erythrocytes. Addition of TiO₂ and ZnO EPs to platelet-rich plasma caused activation of human platelets after 24 hours and 3 hours, respectively, while in canine erythrocytes, activation of platelets due to ZnO EPs occurred already after 1 hour. To assess the effect of EPs on a representative sample of giant unilamelar phospholipid vesicles, analysis of the recorded populations was improved by applying the principles of statistical physics. TiO₂ EPs did not induce any notable effect on giant unilamelar phospholipid vesicles within 50 minutes of incubation, while ZnO EPs induced a decrease in the number of giant unilamelar phospholipid vesicles that was statistically significant (p < 0,001) already after 20 minutes of incubation.

Conclusions

These results indicate that TiO₂ and ZnO EPs cause erythrocyte aggregation and could be potentially prothrombogenic, while ZnO could also cause membrane rupture.

【 授权许可】

   
2013 Šimundić et al.; licensee BioMed Central Ltd.

【 图 表 】

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