The effects of multifunctional MiR-122-loaded graphene-gold composites on drug-resistant liver cancer

Journal of Nanobiotechnology

Gen Zhang³  Nongyue He³  Xin Zeng⁴  Ming Li⁶  Yanjun Kang⁵  Heming Wu⁶  Bin Liu²  Yaqin Zhang¹  Yi Yuan⁶ 

[1] Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Department of Biomedical Engineering, Nanjing Medical University, Nanjing 210029, China;The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;Maternal and Child Health Institute, Nanjing Maternity and Child Health Care Hospital, Nanjing 210029, China;Jiangnan University Medical School, Wuxi 214122, Jiangsu, China;Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China

关键词: Target;    Control release;    Cell apoptosis;    Gold nanoparticles;    Graphene;   

Others  :  1133156
DOI  :  10.1186/s12951-015-0070-z

 received in 2014-11-03, accepted in 2015-01-23,  发布年份 2015

【 摘 要 】

Background

Nano drugs have attracted increased attention due to their unique mode of action that offers tumor-inhibiting effects. Therefore, we have previously explored functionalized and drug-loaded graphene-gold nanocomposites that induced cancer cell apoptosis.

Results

In the present study, we developed a combination of monoclonal P-glycoprotein (P-gp) antibodies, folic acid (FA) and miR-122-loaded gold nanoparticles on graphene nanocomposites (GGMPN), which promoted drug-resistant HepG2 cell apoptosis with drug targeting and controlled release properties. We also investigated related apoptosis proteins and apoptosis signal pathways by GGMPN treatment in vitro and in vivo. Moreover, we further demonstrated the inhibition of tumor growth and the apoptosis-inducing effect by means of GGMPN with a semiconductor laser in a xenograft tumor model.

Conclusion

In conclusion, our results collectively suggested that GGMPN could serve as a novel therapeutic approach to control tumor cell apoptosis and growth.

【 授权许可】

   
2015 Yuan et al.; licensee BioMed Central.

【 图 表 】

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