Increased SGLT1 expression in salivary gland ductal cells correlates with hyposalivation in diabetic and hypertensive rats

Diabetology & Metabolic Syndrome

Ubiratan Fabres Machado²  Helayne Soares Freitas²  Rosana Cristina Mori²  Aline David-Silva²  Maristela Mitiko Okamoto²  Robinson Sabino-Silva¹ 

[1] Institute of Biological Sciences and Health, Federal University of Alagoas (UFAL), Av. Lourival de Melo Mota, km 14, Campus A. C. Simões, Cidade Universitária, Maceió/AL CEP 57072-970, Brazil;Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil

关键词: Submandibular;    Parotid;    Glucose reabsorption;    Xerostomia;    Salivary secretion;   

Others  :  812595
DOI  :  10.1186/1758-5996-5-64

 received in 2013-06-13, accepted in 2013-10-15,  发布年份 2013

【 摘 要 】

Background

Oral health complications in diabetes and hypertension include decreased salivary secretion. The sodium-glucose cotransporter 1 (SGLT1) protein, which transports 1 glucose/2 Na⁺/264 H₂O molecules, is described in salivary glands. We hypothesized that changes in SGLT1 expression in the luminal membrane of ductal cell may be related to an altered salivary flow.

Findings

By immunohistochemistry, we investigated SGLT1 expression in ductal cells of parotid and submandibular glands from Wistar Kyoto rats (WKY), diabetic WKY (WKY-D), spontaneously hypertensive rats (SHR) and diabetic SHR (SHR-D), as well as in parotid glands from WKY subjected to sympathetic stimulation, with or without previous propranolol blockade. Diabetes and hypertension decreased the salivary secretion and increased SGLT1 expression in the luminal membrane of ductal cells, and their association exacerbated the regulations observed. After 30 min of sympathetic stimulation, SGLT1 increased in the luminal membrane of ductal cells, and that was blocked by previous injection of propranolol.

Conclusions

SGLT1 expression increases in the luminal membrane of salivary gland ductal cells and the salivary flow decreases in diabetic and hypertensive rats, which may be related to sympathetic activity. This study highlights the water transporter role of SGLT1 in salivary glands, which, by increasing ductal water reabsorption, may explain the hyposalivation of diabetic and hypertensive subjects.

【 授权许可】

   
2013 Sabino-Silva et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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