Psoriasin (S100A7) is a novel biomarker for lung squamous cell carcinoma in humans

Cancer Cell International

Jihong Zhang³  Xueying Song²  Guilan Liu³  Qiang Wu³  Guijuan Liu¹ 

[1] Department of Cytology Laboratory, People’s Hospital of Linyi, Linyi 276300, Shandong, China;Department of Thoracic Surgery, the People’s Hospital of Rizhao, Rizhao, China;Department of Nursing, People’s Hospital of Yinan, Linyi 276300, Shandong, China

关键词: NF-κB;    S100A7;    Lung squamous cell carcinoma;   

Others  :  1138482
DOI  :  10.1186/s12935-014-0154-0

 received in 2014-09-19, accepted in 2014-12-27,  发布年份 2015

【 摘 要 】

Objective

Psoriasin (S100A7) plays a role in the malignant potential of several epithelial cancers, and could candidate diagnostic marker or therapeutic target. Nuclear factor kappa B (NF-κB) regulates cancer cell growth and is modulated by phospholipase activity in many cancer cells. In the present study, we first evaluate the involvement of S100A7 in lung squamous cell carcinoma and its clinical usefulness for diagnosis. We then study whether knockdown of S100A7 in lung squamous cell carcinoma cells would reduce cell proliferation and NF-κB activity in vitro and attenuate tumor growth in vivo.

Methods

We examined S100A7 expression in lung squamous cell carcinoma tissues by immunohistology. The human lung squamous cell carcinoma cell line NCI-H520 were transduced with short hairpin RNA targeting S100A7. Quantitative reverse transcriptase-polymerase chain reaction and immunoblotting confirmed knockdown of S100A7 messenger RNA and protein, respectively. Cell proliferation was evaluated by the MTT assay. NF-κB phosphorylation was assayed by western blot. 1 × 10⁶ of NCI-H520/S100A7 knockdown cells were injected into the left flanks of nude mice (aged 6 to 8 weeks). Tumors were followed for 35 days, then removed and stained with hematoxylin and eosin, stained with Ki-67, and analyzed for S100A7 protein expression.

Results

S100A7 protein levels were significantly higher in carcinoma specimens than in nonneoplastic tissues. S100A7 might be a useful marker for diagnosis of lung squamous cell carcinoma. In vitro data showed that inhibition of S100A7 decreased proliferation of NCI-H520 cells. S100A7 knockdown reduced NF-κB phosphorylation and tumor growth in vivo and vivo. Explanted knockdown tumors maintained lower S100A7 levels compared with wild-type, confirmed by immunohistology. Ki-67 staining was more prominent throughout the wild-type tumors compared with knockdown tumors.

Conclusions

Our present results suggest that S100A7 level is a promising tool for diagnosis of lung squamous cell carcinoma. Knockdown of S100A7 suppresses lung cancer growth in part by attenuating NF-κB activity. S100A7 may be a promising therapeutic target for lung squamous cell carcinoma.

【 授权许可】

   
2015 Liu et al.; licensee BioMed Central.

【 图 表 】

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