Contribution of the TCF7L2 rs7903146 (C/T) gene polymorphism to the susceptibility to type 2 diabetes mellitus in Cameroon

Journal of Diabetes & Metabolic Disorders

Jean Claude Mbanya⁵  Wilfred F Mbacham³  Folefac Aminkeng²  Marie-Solange Evehe¹  Rosine Djokam-Dadjeu⁴  Eric Djahmeni⁵  Priscille Pokam Fosso¹  Edith Pascale Mato-Mofo¹  Elvis Ndonwi Ngwa¹  Jean Jacques N Noubiap⁶  Olivier Sontsa Donfack¹  Barbara Atogho-Tiedeu¹  Eugene Sobngwi¹  Magellan Guewo-Fokeng¹ 

[1] Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon;The Canadian Pharmacogenomics Network for Drug Safety (CPNDS), Center for Molecular Medicine and Therapeutics, Department of Medical Genetics, University of British Columbia, Vancouver, Canada;Laboratory for Public Health Research Biotechnologies, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon;Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon;National Obesity Center, Yaoundé Central Hospital, University of Yaoundé 1, Yaoundé, Cameroon;Medical Diagnostic Center, Yaoundé, Cameroon

关键词: Cameroon;    Sub-Saharan Africa;    Transcription factor 7-like 2 (TCF7L2);    Genetic association;    Type 2 diabetes;   

Others  :  1173880
DOI  :  10.1186/s40200-015-0148-z

 received in 2014-11-11, accepted in 2015-03-08,  发布年份 2015

【 摘 要 】

Background

Data on the genetic variants for type 2 diabetes mellitus (T2DM) in sub-Saharan African populations are very scarce. This study aimed to investigate the association of transcription factor 7-like (TCF7L2) with T2DM in a Cameroonian population and explore possible genotype-phenotype correlation.

Methods

This is a case–control study involving 37 T2DM patients and 37 non-diabetic volunteers of Cameroonian ethnicity aged 40 years old and above. We collected clinical and biological data to determine phenotypic traits. TCF7L2 was analyzed by genotyping for rs7903146 (C/T) using PCR-RFLP. Biochemical analyses were performed using a spectrophotometer with Chronolab kits. Statistical analyses were carried out using IBM SPSS, PS and Quanto.

Results

TCF7L2 was associated with T2DM in this Cameroonian population (p = 0.013 for alleles, and p = 0.013 for genotypes). The risk allele was C (9.5% patients vs. 0% healthy controls, OR = 16.56) and the protective allele was T (90.5% patients vs. 100.0% healthy controls, OR = 0.06). The risk genotype was C/T (18.9% patients vs. 0% healthy controls, OR = 18.44), while the protective genotype was T/T (81.1% patients vs. 100.0% healthy controls, OR = 0.054). The statistical power was 99.99%. TCF7L2 was not preferentially associated with a specific disease phenotype.

Conclusion

TCF7L2 is associated with T2DM in this Cameroonian population. The association is not dependent on a specific T2DM phenotype. Clinical genetic testing for TCF7L2 can help to predict the occurrence of T2DM in Cameroon.

【 授权许可】

   
2015 Guewo-Fokeng et al.; licensee BioMed Central.

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