Fenugreek with reduced bitterness prevents diet-induced metabolic disorders in rats

Lipids in Health and Disease

Keizo Kasono²  Nobuyo Tsunoda²  Nobuaki Tsuge¹  Shohei Hoshino¹  Keiko Taketani¹  Hiroshige Chiba²  Etsuko Muraki² 

[1] House Foods Corporation, Somatech Center, Chiba, 284-0033, Japan;Department of Clinical Dietetics & Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Saitama, 350-0295, Japan

关键词: Rats;    Diet-induced metabolic disorders;    High-fat high-sucrose diet;    FRB;   

Others  :  1160305
DOI  :  10.1186/1476-511X-11-58

 received in 2012-02-19, accepted in 2012-05-29,  发布年份 2012

【 摘 要 】

Background

Various therapeutic effects of fenugreek (Trigonella foenum-graecum L.) on metabolic disorders have been reported. However, the bitterness of fenugreek makes it hard for humans to eat sufficient doses of it for achieving therapeutic effects. Fenugreek contains bitter saponins such as protodioscin. Fenugreek with reduced bitterness (FRB) is prepared by treating fenugreek with beta-glucosidase. This study has been undertaken to evaluate the effects of FRB on metabolic disorders in rats.

Methods

Forty Sprague–Dawley rats were fed with high-fat high-sucrose (HFS) diet for 12 week to induce mild glucose and lipid disorders. Afterwards, the rats were divided into 5 groups. In the experiment 1, each group (n = 8) was fed with HFS, or HFS containing 2.4% fenugreek, or HFS containing 1.2%, 2.4% and 4.8% FRB, respectively, for 12 week. In the experiment 2, we examined the effects of lower doses of FRB (0.12%, 0.24% and 1.2%) under the same protocol (n = 7 in each groups).

Results

In the experiment 1, FRB dose-dependently reduced food intake, body weight gain, epididymal white adipose tissue (EWAT) and soleus muscle weight. FRB also lowered plasma and hepatic lipid levels and increased fecal lipid levels, both dose-dependently. The Plasma total cholesterol levels (mmol/L) in the three FRB and Ctrl groups were 1.58 ± 0.09, 1.45 ± 0.05*, 1.29 ± 0.07* and 2.00 ± 0.18, respectively (*; P < 0.05 vs. Ctrl). The Hepatic total cholesterol levels (mmol/g liver) were 0.116 ± 0.011, 0.112 ± 0.006, 0.099 ± 0.007* and 0.144 ± 0.012, respectively (*; P < 0.05 vs. Ctrl). The calculated homeostasis model assessment as an index of insulin resistance (HOMA-IR) indicated 0.52 ± 0.04*, 0.47 ± 0.06*, 0.45 ± 0.05* and 1.10 ± 0.16, respectively (*; P < 0.05 vs. Ctrl). None of the FRB groups showed any adverse effect on the liver, kidney or hematological functions. In the experiment 2, no significant difference of food intake was observed, while the 1.2% FRB group alone showed nearly the same effects on glucose and lipid metabolism as in the experiment 1.

Conclusions

Thus we have demonstrated that FRB (1.2 ~ 4.8%) prevents diet-induced metabolic disorders such as insulin resistance, dyslipidemia and fatty liver.

【 授权许可】

   
2012 Muraki et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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【 参考文献 】

• [1]Srinivasan K: Fenugreek (Trigonella foenum-graecum): A review of health beneficial physiological effects. Food Rev Int 2006, 22:203-224.
• [2]Reddy RL, Srinivasan K: Dietary fenugreek seed regresses preestablished cholesterol gallstones in mice. Can J Physiol Pharmacol 2009, 87:684-693.
• [3]Reddy RL, Srinivasan K: Fenugreek seeds reduce atherogenic diet-induced cholesterol gallstone formation in experimental mice. Can J Physiol Pharmacol 2009, 87:933-943.
• [4]Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ: Trigonellafoenum graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem 2001, 224:45-51.
• [5]Mohamad S, Taha A, Bamezai RN, Basir SF, Baquer NZ: Lower doses of vanadate in combination with trigonella restore altered carbohydrate metabolism and antioxidant status in alloxan-diabetic rats. Clin Chim Acta 2004, 342:105-114.
• [6]Gad MZ, El-Sawalhi MM, Ismail MF, El-Tanbouly ND: Biochemical study of the anti-diabetic action of the Egyptian plants Fenugreek and Balanites. Mol Cell Biochem 2006, 281:173-183.
• [7]Broca C, Gross R, Petit P, Sauvaire Y, Manteghetti M, Tournier M, Masiello P, Gomis R, Ribes G: 4-Hydroxyisoleucine: experimental evidence of its insulinotropic and antidiabetic properties. Am J Physiol 1999, 277:E617-E623.
• [8]Jette L, Harvey L, Eugeni K, Levens N: 4-Hydroxyisoleucine: a plant-derived treatment for metabolic syndrome. Curr Opin Investig Drugs 2009, 10:353-358.
• [9]Srichamroen A, Thomson AB, Field CJ, Basu TK: In vitro intestinal glucose uptake is inhibited by galactomannan from Canadian fenugreek seed (Trigonella foenum graecum L) in genetically lean and obese rats. Nutr Res 2009, 29:49-54.
• [10]Srichamroen A, Field CJ, Thomson AB, Basu TK: The modifying effects of galactomannan from Canadian-grown fenugreek (Trigonella foenum-graecum L.) on the glycemic and lipidemic status in rats. J Clin Biochem Nutr 2008, 43:167-174.
• [11]Muraki E, Chiba H, Tsunoda N, Kasono K: Fenugreek improves diet-induced metabolic disorders in rats. Horm Metab Res 2011, 43:950-955.
• [12]Muraki E, Hayashi Y, Chiba H, Tsunoda N, Kasono K: Dose-dependent effects, safety and tolerability of fenugreek in diet-induced metabolic disorders in rats. Lipids Health Dis 2011, 10:240. BioMed Central Full Text
• [13]Masamura N, Sawada H: Regarding bitter components in fenugreek (Trigonella foenum-graecum L.) seeds. Abstract of the 14 th Annual Meeting of Japan Society for Spice Research (Nihon Koshinryo Kenkyukai koen yoshisu) 1999, 22-23. cf. Preparing additional file
• [14]Uemura T, Hirai S, Mizoguchi N, Goto T, Lee JY, Taketani K, Nakano Y, Shono J, Hoshino S, Tsuge N, Narukami T, Takahashi N, Kawada T: Diosgenin present in fenugreek improves glucose metabolism by promoting adipocyte differentiation and inhibiting inflammation in adipose tissues. Mol Nutr Food Res 2010, 54:1596-1608.
• [15]Reeves PG, Nielsen FH, Fahey GC: AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993, 123:1939-1951.
• [16]Lee SC, Rodriguez F, Storey M, Farmakalidis E, Prosky L: Determination of soluble and insoluble dietary fiber in psyllium-containing cereal products. J AOAC Int 1995, 78:724-729.
• [17]Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959, 37:911-917.
• [18]Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957, 226:497-509.
• [19]Anderson JW, Jones AE, Riddell-Mason S: Ten different dietary fibers have significantly different effects on serum and liver lipids of cholesterol-fed rats. J Nutr 1994, 124:78-83.
• [20]Wong JM, de Souza R, Kendall CW, Emam A, Jenkins DJ: Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol 2006, 40:235-243.
• [21]Comalada M, Bailón E, de Haro O, Lara-Villoslada F, Xaus J, Zarzuelo A, Gálvez JM: The effects of short-chain fatty acids on colon epithelial proliferation and survival depend on the cellular phenotype. J Cancer Res Clin Oncol 2006, 132:487-497.
• [22]Casterline JL, Oles CJ, Ku Y: In vitro fermentation of various food fiber fractions. J Agr Food Chem 1997, 45:2463-2467.
• [23]Ebihara K, Masuhara R, Kiriyama S: Major determinants of plasma glucose-flattening activity of a water-soluble dietary fiber: Effects of konjac mannan on gastric emptying and intraluminal glucose-diffusion. Nutr Rep Int 1981, 23:1145-1156.
• [24]Hirai S, Uemura T, Mizoguchi N, Lee JY, Taketani K, Nakano Y, Hoshino S, Tsuge N, Narukami T, Yu R, Takahashi N, Kawada T: Diosgenin attenuates inflammatory changes in the interaction between adipocytes and macrophages. Mol Nutr Food Res 2010, 54:797-804.
• [25]Ramadan G, El-Beih NM, Abd El-Kareem HF: Anti-metabolic syndrome and immunostimulant activities of Egyptian fenugreek seeds in diabetic/obese and immunosuppressive rat models. Br J Nutr 2010, 23:1-10.
• [26]Reddy RR, Srinivasan K: Effect of dietary fenugreek seeds on biliary proteins which influence nucleation of cholesterol crystals in bile. Steroids 2011, 76:455-463.
• [27]Uemura T, Goto T, Kang MS, Mizoguchi N, Hirai S, Lee JY, Nakano Y, Shono J, Hoshino S, Taketani K, Tsuge N, Narukami T, Makishima M, Takahashi N, Kawada T: Diosgenin, the main aglycon of fenugreek, inhibits LXRα activity in HepG2 cells and decreases plasma and hepatic triglycerides in obese diabetic mice. J Nutr 2011, 141:17-23.