Exercise reduces activation of microglia isolated from hippocampus and brain of aged mice

Journal of Neuroinflammation

Justin S Rhodes²  Elzbieta Wojcik²  Tushar K Bhattacharya²  Rachel A Kohman¹ 

[1] Department of Psychology, University of North Carolina Wilmington, 601 S. College Road, Wilmington, NC 28403-5612, USA;Department of Psychology, University of Illinois at Urbana-Champaign, Beckman Institute, Urbana, IL 61801-3873, USA

关键词: CD206;    CD86;    MHC II;    Microglia;    Aging;    Hippocampus;    Exercise;   

Others  :  1152125
DOI  :  10.1186/1742-2094-10-114

 received in 2013-05-17, accepted in 2013-09-06,  发布年份 2013

【 摘 要 】

Background

Aging is associated with low-grade neuroinflammation that includes basal increases in proinflammatory cytokines and expression of inflammatory markers on microglia. Exercise can reduce neuroinflammation following infection in aged animals, but whether exercise modulates basal changes in microglia activation is unknown. Therefore, we evaluated changes in basal microglia activation in cells isolated from the hippocampus and remaining brain following running-wheel access.

Methods

Adult (4 months) and aged (22 months) male and female BALB/c mice were housed with or without running wheels for 10 weeks. Microglia were isolated from the hippocampus or remaining brain. Flow cytometry was used to determine microglia (CD11b+ and CD45^low) that co-labeled with CD86, CD206, and MHC II.

Results

Aged mice showed a greater proportion of CD86 and MHC II positive microglia. In aged females, access to a running wheel decreased proportion of CD86+ and MHC II+ microglia in the hippocampus whereas aged males in the running group showed a decrease in the proportion of CD86+ microglia in the brain and an increase in the proportion of MHC II+ microglia in hippocampus and brain.

Conclusion

Overall, these data indicate that running-wheel access modulates microglia activation, but these effects vary by age, sex, and brain region.

【 授权许可】

   
2013 Kohman et al.; licensee BioMed Central Ltd.

【 图 表 】

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