Characterisation of interleukin-10 expression on different vascular structures in allergic nasal mucosa

Clinical and Translational Allergy

Cornelis M van Drunen¹  Wytske J Fokkens¹  Esther JJ de Groot¹  Danielle van Egmond¹  Barbara Muller¹ 

[1] Department of Otorhinolaryngology, AMC, Room L3-104-2, Meibergdreef 9, Amsterdam 1100 DD, The Netherlands

关键词: Nasal allergen provocation;    Nasal endothelium;    Leukocyte trafficking;    Interleukin-10;    Blood vessels;    Allergic rhinitis;    Adhesion molecules;   

Others  :  794088
DOI  :  10.1186/2045-7022-4-2

 received in 2013-07-31, accepted in 2013-12-20,  发布年份 2014

【 摘 要 】

Background

Interleukin-10 (IL-10) is a negative regulator of immune responses and was previously shown to be expressed by human nasal endothelial cells, while the adhesion molecule MECA-79 plays a role in trans-endothelial migration of immune competent cells. In this study we investigate the relationship between endothelial IL-10 and MECA-79 expression to address the question whether immune competent cells could be affected at the mucosal entry site.

Methods

Nasal turbinate biopsies were taken from house dust mite allergic patients, before and after nasal allergen provocation. Subsequent slides of biopsies were stained for IL10, MECA-79, CD34, and IL10-Receptor. Capillaries, arteries/veins, and sinusoids were evaluated separately.

Results

90% of sinusoids are IL-10 positive and all sinusoids are negative for MECA-79, while 4.8% of capillaries are positive for IL-10, and 2.2% are positive for MECA-79. Although about 47% of arteries/veins are positive for IL-10 and 57.1% are positive for MECA-79, only about 20% are positive for both markers. Furthermore, we showed that the myo-fibroblasts surrounding all sinusoids stain positive for IL10R.

Conclusions

IL10 expression on vascular structures is not related to MECA expression for sinusoids and capillaries and only partly related on arteries/veins, however sinusoidal endothelial IL10 expression is always seen in combination with IL-10R expression of sinusoidal myo-fibroblasts.

【 授权许可】

   
2014 Muller et al.; licensee BioMed Central Ltd.

【 图 表 】

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