Biodegradation of naphthalene using Pseudomonas aeruginosa by up flow anoxic–aerobic continuous flow combined bioreactor

Journal of Environmental Health Science Engineering

Mehry Esvand¹  Maryam Habibi¹  Behrooz Karimi² 

[1] Department of Environmental Health Engineering, School of Health, Arak University of Medical Sciences, Arak, Iran;Department of Environmental Health Engineering, School of Health, Tehran University of Medical Sciences, Tehran, Iran

关键词: Pseudomonas aeruginosa;    Naphthalene;    Biodegradation;    Anoxic–aerobic continuous flow combined bioreactor;   

Others  :  1161179
DOI  :  10.1186/s40201-015-0175-1

 received in 2014-10-25, accepted in 2015-03-03,  发布年份 2015

【 摘 要 】

Background

Naphthalene is a poly aromatic hydrocarbon (PAH) present in many sediment-water systems. The aim of this study was to evaluate the applicability of an anoxic/aerobic system for the biological treatment of water polluted by naphthalene by Pseudomonas aeruginosa PTCC 1707 to utilize naphthalene. The naphthalene elimination from wastewater was determined in anoxic–aerobic continuous flow combined bioreactor under continuously oxic and anoxic conditions. Experiments were conducted in continues mode, and naphthalene was administered in consecutive spike doses. Then Pseudomonas aeruginosa bacteria suspension with a specific turbidity (0.5-10 NTU) was prepared from growing bacteria on R₂A medium and injected to reactor.

Findings

At naphthalene concentration = 0.5-20 mg/L, 33–65.5% naphthalene removal efficiencies were observed. Mean COD removal efficiency in solid retention times of 2, 4, 6, and 8 days was 82.7, 92.45, 95.97 and 96.1%, respectively. Naphthalene removal efficiency by bacterium pseudomonas at pH 8 was 96% and at pH 4, 5.5, 7 and 9.5, 68, 80, 90 and 85%, respectively. As the initial concentration of naphthalene increased from 0.5 to 20 mg/L, the remaining concentration of naphthalene decreased from 33.4% to 65.5% after 3 days.

Conclusion

Based on experimental results, it was determined that this process can effectively reduce naphthalene under optimal conditions and this method can be used for the removal of similar compounds.

【 授权许可】

   
2015 Karimi et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

Figure 7.

Figure 8.

【 参考文献 】

• [1]Darvishi Cheshmeh Soltani R, Rezaee A, Safari M, Khataee AR, Karimi B: Photocatalytic degradation of formaldehyde in aqueous solution using ZnO nanoparticles immobilized on glass plates. Desalin Water Treat 2013, 53:1613-1620.
• [2]Anbia M, Moradi SE: Removal of naphthalene from petrochemical wastewater streams using carbon nanoporous adsorbent. Appl Surf Sci 2009, 255:5041-7.
• [3]Karimi B, Ehrampoush MH, Ebrahimi A, Mokhtari M, Amin MM: Catalytic oxidation of hydrogen peroxide and the adsorption combinatory process in leachate waste pretreatment from composting factory. Int J Environ Health Eng 2012, 1:15.
• [4]Aranda A, López JM, Murillo R, Mastral AM, Dejoz A, Vázquez I: Total oxidation of naphthalene with high selectivity using a ceria catalyst prepared by a combustion method employing ethylene glycol. J Hazard Mater 2009, 171:393-9.
• [5]Di Gennaro P, Moreno B, Annoni E, García-Rodríguez S, Bestetti G, Benitez E: Dynamic changes in bacterial community structure and in naphthalene dioxygenase expression in vermicompost-amended PAH-contaminated soils. J Hazard Mater 2009, 172:1464-9.
• [6]Nesterenko-Malkovskaya A, Kirzhner F, Zimmels Y, Armon R: Eichhornia crassipescapability to remove naphthalene from wastewater in the absence of bacteria. Chemosphere 2012, 125:124-8.
• [7]Badr T, Hanna K, De Brauer C: Enhanced solubilization and removal of naphthalene and phenanthrene by cyclodextrins from two contaminated soils. J Hazard Mater 2004, 112:215-23.
• [8]Gu L, Nie JY, Zhu N, Wang L, Yuan HP, Shou Z: Enhanced Fenton’s degradation of real naphthalene dye intermediate wastewater containing 6-nitro-1-diazo-2-naphthol-4-sulfonic acid: A pilot scale study. Chem Eng J 2012, 45:22-8.
• [9]Chang CF, Chang CY, Chen KH, Tsai WT, Shie JL, Chen YH: Adsorption of naphthalene on zeolite from aqueous solution. J Colloid Interface Sci 2004, 277:29-34.
• [10]Karimi B, Rajaei M-S, Ganadzadeh MJ, Mashayekhi M, Jahanbakhsh M: Evaluation of nitrate removal from water by Fe/H2O2 and adsorption on activated carbon. Arak Med Univ J 2013, 15:67-76.
• [11]Akhtar S, Husain Q: Potential applications of immobilized bitter gourd (Momordica charantia) peroxidase in the removal of phenols from polluted water. Chemosphere 2006, 65:1228-35.
• [12]Soberon-Chavez G, Lepine F, Deziel E: Production of rhamnolipids by Pseudomonas aeruginosa. Appl Microbiol Biotechnol 2005, 68:718-25.
• [13]Goel RK, Flora JR, Ferry J: Mechanisms for naphthalene removal during electrolytic aeration. Water Res 2003, 37:891-901.
• [14]Karimi B, Ehrampoush MH, Ebrahimi A, Mokhtari M: The study of leachate treatment by using three advanced oxidation process based wet air oxidation. Iran J Environ health Sci Eng 2013, 10:1-7. BioMed Central Full Text
• [15]Maillacheruvu K, Safaai S: Naphthalene removal from aqueous systems by Sagittarius sp. J Environ Sci Health, Part A 2002, 37:845-61.
• [16]Pathak H, Kantharia D, Malpani A, Madamwar D: Naphthalene degradation by < i > Pseudomonas sp. HOB1: In vitro studies and assessment of naphthalene degradation efficiency in simulated microcosms. J Hazard Mater 2009, 166:1466-73.
• [17]Beklemishev M, Kozliak E: Bioremediation of concrete contaminated with n‐hexadecane and naphthalene. Acta biotechnologica 2003, 23:197-210.
• [18]Seoud MA, Maachi R: Biodegradation of naphthalene by free and alginate entrapped Pseudomonas sp. Zeitschrift Fur Naturforsch C 2003, 58:726-31.
• [19]Kozlova E, Puntus I, Slepenkin A, Boronin A: Naphthalene degradation by Pseudomonas putida strains in soil model systems with arsenite. Process Biochem 2004, 39:1305-8.
• [20]Feijoo-Siota L, Rosa-Dos-Santos F, de Miguel T, Villa T: Biodegradation of Naphthalene by Pseudomonas stutzeri in Marine Environments: Testing Cells Entrapment in Calcium Alginate for Use in Water Detoxification. Bioremediation J 2008, 12:185-92.
• [21]Quantin C, Joner E, Portal J, Berthelin J: PAH dissipation in a contaminated river sediment under oxic and anoxic conditions. Environ Pollut 2005, 134:315-22.
• [22]Roslev P, King GM: Survival and recovery of methanotrophic bacteria starved under oxic and anoxic conditions. Appl Environ Microbiol 1994, 60:2602-8.
• [23]W-t Z, Huang X, Lee D-J: Enhanced treatment of coke plant wastewater using an anaerobic–anoxic–oxic membrane bioreactor system. Sep Purif Technol 2009, 66:279-86.
• [24]Karimi B, Ehrampoush MH, Jabary H: Indicator pathogens, organic matter and LAS detergent removal from wastewater by constructed subsurface wetlands. J Environ Health Sci Eng 2014, 12:52-9. BioMed Central Full Text
• [25]Tuomi PM, Salminen JM, Jørgensen KS: The abundance of nahAc genes correlates with the 14C‐naphthalene mineralization potential in petroleum hydrocarbon‐contaminated oxic soil layers. FEMS Microbiol Ecol 2004, 51:99-107.
• [26]Chang BV, Chang I, Yuan SY: Biodegradation of phenanthrene and pyrene from mangrove sediment in subtropical Taiwan. J Environ Sci Health Part A 2008, 43:233-8.
• [27]Blum P, Hunkeler D, Weede M, Beyer C, Grathwohl P, Morasch B: Quantification of biodegradation for xylene and naphthalene using first order decay models, Michaelis–Menten kinetics and stable carbon isotopes. J Contam Hydrol 2009, 105:118-30.
• [28]Wen Y, Ren Y, Wei C-H, Li K-Y, Lin F-M, Chen X-Y: A study on nitrogen removal efficiency of Pseudomonas stutzeri strains isolated from an anaerobic/anoxic/oxic wastewater treatment process. Afr J Biotechnol 2010, 9:869-73.
• [29]Sun HM, Tian WJ, Wang YM: Occurrence and Fate of Polycyclic Aromatic Hydrocarbons in the Anaerobic-Anoxic-Oxic Wastewater Treatment Process. Adv Mate Res 2013, 610:1722-5.