Correlative Imaging of Structural and Elemental Composition of Bacterial Biofilms

11th International Conference on X-ray Microscopy

Yang, Y.^1,5 ; Heine, R.^1 ; Xu, F.^1 ; Suhonen, H.^2 ; Helfen, L.^1,2 ; Rosenhahn, A.^3,4 ; Gorniak, T.^3,4 ; Kirchen, S.^4 ; Schwartz, T.^4 ; Baumbach, T.^1

Institute for Photon Science and Synchrotron Radiation, ANKA, Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany^1

European Synchrotron Radiation Facility (ESRF), 38043 Grenoble, France^2

Analytical Chemistry-Biointerfaces, University of Bochum, 44801Bochum, Germany^3

Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany^4

Institute of Geoscience, University of Freiburg, 79104 Freiburg, Germany^5

关键词: Bacterial biofilm;    Elemental compositions;    Elemental contents;    Elemental distribution;    Microbial communities;    Phase contrast tomography;    Pseudomonas aeruginosa;    Threedimensional (3-d);   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012053/pdf
DOI  :  10.1088/1742-6596/463/1/012053

来源: IOP

【 摘 要 】

Synchrotron-based phase contrast tomography (holotomography) and scanning hard X-ray fluorescence microscopy (SXFM) are combined to characterize the three-dimensional (3D) structural and corresponding elemental distribution of bacterial biofilms of Pseudomonas aeruginosa. Samples were fixed without contrast agents or microtomal sectioning. Within an intact microbial community single bacteria are clearly resolved, and their morphology can be directly visualized together with the elemental content. Such 3D set of complementary information at cellular level is essential for gaining a deeper understanding of biofilm evolution aiming to develop potential strategies on biofilm growth control and prevention.