Metabolic profile response to administration of epigallocatechin-3-gallate in high-fat-fed mice

Diabetology & Metabolic Syndrome

Lila Missae Oyama³  Claudia Maria Oller do Nascimento³  Eliane Beraldi Ribeiro³  Monica Marques Telles⁴  Cláudio Teodoro de Souza⁵  Gabriel Inácio de Morais Honorato de Souza³  Fábio Santos Lira¹  Marcos Hiromu Okuda³  Rachel De Laquila²  Mayara Franzoi Moreno³ 

[1] Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Presidente Prudente, SP, Brazil;Faculdades Integradas Coração de Jesus – FAINC, Santo André, SP, Brasil;Departamento de Fisiologia, Universidade Federal de São Paulo-EPM, São Paulo, SP, Brasil;Departamento de Ciências Biológicas, Universidade Federal de São Paulo-Campus Diadema, Diadema, SP, Brasil;Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil

关键词: Inflammation;    Adipose tissue;    Adipokines;    High-fat diet;   

Others  :  1115079
DOI  :  10.1186/1758-5996-6-84

 received in 2014-01-27, accepted in 2014-08-06,  发布年份 2014

【 摘 要 】

Background

Obesity is associated with increased adipose tissue and glucose intolerance. High-fat diets (HFDs) are known to induce obesity and increase proinflammatory adipokines. The consumption of green tea may improve the health of obese individuals because it contains a potent antioxidant that has effects on body weight, energy expenditure and serum cholesterol concentrations.

Methods

We examined the effects of epigallocatechin-3-gallate (EGCG) (50 mg/kg body weight per day) or saline after 30 or 60 days of treatment. Mice were distributed into four groups: 1) NS: normolipidic diet receiving saline; 2) NE: normolipidic diet receiving EGCG; 3) HFS: high-fat diet receiving saline; 4) HFE: high-fat diet receiving EGCG.

Results

We observed that administration of a HFD plus EGCG treatment for 60 days reduced delta weight, the relative weights of the mesenteric adipose tissue (MES), retroperitonial adipose tissue (RET), epididymal adipose tissue (EPI), the sum of the adipose tissues (SAT), reduced triacylglycerol (TG) and improved both high-density lipoprotein (HDL) cholesterol levels and the adiponectin/STA ratio when compared with HFS.

Conclusions

Our results suggest that the chronic administration of EGCG (60 days) promoted a significant improvement in glucose tolerance, decreased adipose tissue deposits, weight mass, TG and HDL-C only when associated with high-fat diet treatment.

【 授权许可】

   
2014 Moreno et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Jequier E: Pathways to obesity. Int J Obes Relat Metab Disord 2002, 26(Suppl 2):S12-17.
• [2]Yamashita AS, Lira FS, Rosa JC, Paulino EC, Brum PC, Negrao CE, dos Santos RV, Batista ML Jr, do Nascimento CO, Oyama LM, Seelaender M: Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: the effect of aerobic training and energy restriction. Cytokine 2010, 52:168-174.
• [3]Nascimento CM O d, Ribeiro EB, Oyama LM: Metabolism and secretory function of white adipose tissue: effect of dietary fat. An Acad Bras Cienc 2009, 81:453-466.
• [4]Trayhurn P: Endocrine and signalling role of adipose tissue: new perspectives on fat. Acta Physiol Scand 2005, 184:285-293.
• [5]Lira FS, Rosa JC, Cunha CA, Ribeiro EB, do Nascimento CO, Oyama LM, Mota JF: Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3 T3-L1 adipocytes following LPS stimulation. Lipids Health Dis 2011, 10:37.
• [6]Sae-Tan S, Grove KA, Kennett MJ, Lambert JD: (-)-Epigallocatechin-3-gallate increases the expression of genes related to fat oxidation in the skeletal muscle of high fat-fed mice. Food function 2011, 2:111-116.
• [7]Klaus S, Pultz S, Thone-Reineke C, Wolfram S: Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes (Lond) 2005, 29:615-623.
• [8]Thielecke F, Boschmann M: The potential role of green tea catechins in the prevention of the metabolic syndrome - a review. Phytochemistry 2009, 70:11-24.
• [9]Yang CS, Wang X, Lu G, Picinich SC: Cancer prevention by tea: animal studies, molecular mechanisms and human relevance. Nat Rev Cancer 2009, 9:429-439.
• [10]Venables MC, Hulston CJ, Cox HR, Jeukendrup AE: Green tea extract ingestion, fat oxidation, and glucose tolerance in healthy humans. Am J Clin Nutr 2008, 87:778-784.
• [11]Friedrich M, Petzke KJ, Raederstorff D, Wolfram S, Klaus S: Acute effects of epigallocatechin gallate from green tea on oxidation and tissue incorporation of dietary lipids in mice fed a high-fat diet. Int J Obes (Lond) 2012, 36:735-743.
• [12]Li Y, Zhao S, Zhang W, Zhao P, He B, Wu N, Han P: Epigallocatechin-3-O-gallate (EGCG) attenuates FFAs-induced peripheral insulin resistance through AMPK pathway and insulin signaling pathway in vivo. Diabetes Res Clin Pract 2011, 93:205-214.
• [13]Reeves PG: Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 1997, 127:838S-841S.
• [14]Rankinen T, Zuberi A, Chagnon YC, Weisnagel SJ, Argyropoulos G, Walts B, Perusse L, Bouchard C: The human obesity gene map: the 2005 update. Obesity (Silver Spring) 2006, 14:529-644.
• [15]Shen CL, Chyu MC, Pence BC, Yeh JK, Zhang Y, Felton CK, Doctolero S, Wang JS: Green tea polyphenols supplementation and Tai Chi exercise for postmenopausal osteopenic women: safety and quality of life report. BMC Compl Alternative Med 2010, 10:76.
• [16]Shen CL, Yeh JK, Samathanam C, Cao JJ, Stoecker BJ, Dagda RY, Chyu MC, Dunn DM, Wang JS: Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation. Osteoporos Int 2011, 22:327-337.
• [17]Boschmann M, Thielecke F: The effects of epigallocatechin-3-gallate on thermogenesis and fat oxidation in obese men: a pilot study. J Am Coll Nutr 2007, 26:389S-395S.
• [18]Lonac MC, Richards JC, Schweder MM, Johnson TK, Bell C: Influence of short-term consumption of the caffeine-free, epigallocatechin-3-gallate supplement, Teavigo, on resting metabolism and the thermic effect of feeding. Obesity (Silver Spring) 2011, 19:298-304.
• [19]Esposito K, Nappo F, Giugliano F, Di Palo C, Ciotola M, Barbieri M, Paolisso G, Giugliano D: Meal modulation of circulating interleukin 18 and adiponectin concentrations in healthy subjects and in patients with type 2 diabetes mellitus. Am J Clin Nutr 2003, 78:1135-1140.
• [20]Xydakis AM, Case CC, Jones PH, Hoogeveen RC, Liu MY, Smith EO, Nelson KW, Ballantyne CM: Adiponectin, inflammation, and the expression of the metabolic syndrome in obese individuals: the impact of rapid weight loss through caloric restriction. J Clin Endocrinol Metab 2004, 89:2697-2703.
• [21]Marcell TJ, McAuley KA, Traustadottir T, Reaven PD: Exercise training is not associated with improved levels of C-reactive protein or adiponectin. Metabolism 2005, 54:533-541.
• [22]Tiikkainen M, Bergholm R, Vehkavaara S, Rissanen A, Hakkinen AM, Tamminen M, Teramo K, Yki-Jarvinen H: Effects of identical weight loss on body composition and features of insulin resistance in obese women with high and low liver fat content. Diabetes 2003, 52:701-707.
• [23]Shirai N, Suzuki H: Effects of simultaneous intakes of fish oil and green tea extracts on plasma, glucose, insulin, C-peptide, and adiponectin and on liver lipid concentrations in mice fed low- and high-fat diets. Ann Nutr Metab 2008, 52:241-249.
• [24]Zhou JR, Li L, Pan W: Dietary soy and tea combinations for prevention of breast and prostate cancers by targeting metabolic syndrome elements in mice. Am J Clin Nutr 2007, 86:s882-888.
• [25]Nicklas BJ, You T, Pahor M: Behavioural treatments for chronic systemic inflammation: effects of dietary weight loss and exercise training. CMAJ 2005, 172:1199-1209.
• [26]Fu Z, Zhen W, Yuskavage J, Liu D: Epigallocatechin gallate delays the onset of type 1 diabetes in spontaneous non-obese diabetic mice. Br J Nutr 2011, 105:1218-1225.
• [27]Wellen KE, Hotamisligil GS: Inflammation, stress, and diabetes. J Clin Invest 2005, 115:1111-1119.