Metabolomics of Ramadan fasting: an opportunity for the controlled study of physiological responses to food intake

Journal of Translational Medicine

Karsten Suhre²  Hans Hauner⁵  Jerzy Adamski⁶  Werner Römisch-Margl²  Gabi Kastenmüller²  Joel A Malek⁴  Cornelia Prehn⁷  Anna Artati⁷  Antonia Stank²  Anna Halama⁷  Thomas Skurk⁵  Susanne Krug³  Sweety Mathew¹ 

[1] Department of Physiology and Biophysics, Weill Cornell Medical College – Qatar, Doha, Qatar;Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany;Else Kröner-Fresenius-Centre for Nutritional Medicine, Klinikum rechts der Isar, Technische Universität München, München, Germany;Genomics Core, Weill Cornell Medical College – Qatar, Doha, Qatar;ZIEL - Research Centre for Nutrition and Food Sciences, Technische Universität München, Freising-Weihenstephan, Germany;Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, Germany;Institute of Experimental Genetics, Genome Analysis Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

关键词: Clinical research;    Study design;    Ramadan fasting;    Nutritional challenging;    Metabolomics;   

Others  :  810552
DOI  :  10.1186/1479-5876-12-161

 received in 2014-03-27, accepted in 2014-05-28,  发布年份 2014

【 摘 要 】

High-throughput screening techniques that analyze the metabolic endpoints of biological processes can identify the contributions of genetic predisposition and environmental factors to the development of common diseases. Studies applying controlled physiological challenges can reveal dysregulation in metabolic responses that may be predictive for or associated with these diseases. However, large-scale epidemiological studies with well controlled physiological challenge conditions, such as extended fasting periods and defined food intake, pose logistic challenges. Culturally and religiously motivated behavioral patterns of life style changes provide a natural setting that can be used to enroll a large number of study volunteers. Here we report a proof of principle study conducted within a Muslim community, showing that a metabolomics study during the Holy Month of Ramadan can provide a unique opportunity to explore the pre-prandial and postprandial response of human metabolism to nutritional challenges. Up to five blood samples were obtained from eleven healthy male volunteers, taken directly before and two hours after consumption of a controlled meal in the evening on days 7 and 26 of Ramadan, and after an over-night fast several weeks after Ramadan. The observed increases in glucose, insulin and lactate levels at the postprandial time point confirm the expected physiological response to food intake. Targeted metabolomics further revealed significant and physiologically plausible responses to food intake by an increase in bile acid and amino acid levels and a decrease in long-chain acyl-carnitine and polyamine levels. A decrease in the concentrations of a number of phospholipids between samples taken on days 7 and 26 of Ramadan shows that the long-term response to extended fasting may differ from the response to short-term fasting. The present study design is scalable to larger populations and may be extended to the study of the metabolic response in defined patient groups such as individuals with type 2 diabetes.

【 授权许可】

   
2014 Mathew et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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