Expression of common fragile sites in two Ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)

Genetics Selection Evolution

Marta Mudry²  María Gorostiaga²  Ariela Fundia¹ 

[1] Department of Genetics, Hematology Research Institute, "Mariano R. Castex", National Academy of Medicine, Pacheco de Melo 3081, C.P. 1425, Buenos Aires, Argentina;Department of Biology (GIBE), FCEyN, Buenos Aires University, Buenos Aires, Argentina

关键词: evolution;    heterochromatin;    chromosomal rearrangements;    fragile sites;    Ceboidea;   

Others  :  1094841
DOI  :  10.1186/1297-9686-32-1-87

 received in 1998-09-07, accepted in 1999-11-22,  发布年份 2000

【 摘 要 】

Fragile sites are points of preferential breakage that may be involved in chromosome rearrangements. Induction of common fragile sites (c-fra) and spontaneous breakage were analyzed in two New World Monkeys species: Saimiri boliviensis (SBO) and Alouatta caraya (ACA). Spontaneous chromosome aberrations were analyzed on untreated lymphocyte cultures with Brögger's formula (1977). SBO presented a low level of spontaneous breakage, while higher frequencies were detected in ACA in which bands 1q23; 2q13 and 11q19 were significantly affected (p < 0.01). The populational distribution of c-fra was analyzed by the Chi² test in FUdR plus caffeine treated cultures. A total of 21 c-fra was identified in SBO and 24 in ACA. Fragile sites A₁q33, B₁p21, B₄p14, C₃q23 and C₅q22 were identified in all analyzed SBO specimens. The most frequent c-fra identified in ACA specimens were 1q23, 1q31, 1q33, 2q22, 8q14, 12q31, 13q22, 14q15 and Xq22. Fragile sites A₁q31, A₁q33, B₁q14, B₃q13, B₄q21 and Xq22 identified in SBO and 1q31, 1q33, 2q22, 4q21, 6q13, 13q22 and Xq22 from ACA were the most conserved sites. A low coincidence between the location of c-fra and that of heterochromatin and breakpoints involved in euchromatic rearrangements known for these genera, was established.

【 授权许可】

   
2000 INRA, EDP Sciences