Turnover of multiple sex chromosomes in Harttia catfish (Siluriformes, Loricariidae): a glimpse from whole chromosome painting

Frontiers in Genetics

Genetics

Marcelo Ricardo Vicari¹  Thomas Liehr²  Alexandr Sember³  Marcelo de Bello Cioffi⁴  Geize Aparecida Deon⁴  Francisco de Menezes Cavalcante Sassi⁴  Luiz Antonio Carlos Bertollo⁴  Orlando Moreira Filho⁴  Osvaldo Takeshi Oyakawa⁵ 

[1] Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, Brazil;Institut für Humangenetik, Universitätsklinikum Jena, Jena, Germany;Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czechia;Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil;Museu de Zoologia, Universidade de São Paulo, São Paulo, Brazil;

关键词: microdissection;    WCP;    chromosomal rearrangements;    karyotype;    evolution;   

DOI  :  10.3389/fgene.2023.1226222

 received in 2023-05-20, accepted in 2023-07-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

The remarkable fish biodiversity encompasses also great sex chromosome variability. Harttia catfish belong to Neotropical models for karyotype and sex chromosome research. Some species possess one of the three male-heterogametic sex chromosome systems, XY, X1X2Y or XY1Y2, while other members of the genus have yet uncharacterized modes of sex determination. Particularly the XY1Y2 multiple sex chromosome system shows a relatively low incidence among vertebrates, and it has not been yet thoroughly investigated. Previous research suggested two independent X-autosome fusions in Harttia which led to the emergence of XY1Y2 sex chromosome system in three of its species. In this study, we investigated evolutionary trajectories of synteny blocks involved in this XY1Y2 system by probing six Harttia species with whole chromosome painting (WCP) probes derived from the X (HCA-X) and the chromosome 9 (HCA-9) of H. carvalhoi. We found that both painting probes hybridize to two distinct chromosome pairs in Amazonian species, whereas the HCA-9 probe paints three chromosome pairs in H. guianensis, endemic to Guyanese drainages. These findings demonstrate distinct evolutionary fates of mapped synteny blocks and thereby elevated karyotype dynamics in Harttia among the three evolutionary clades.

【 授权许可】

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Copyright © 2023 Sassi, Deon, Sember, Liehr, Oyakawa, Moreira Filho, Bertollo, Vicari and Cioffi.