Herpesviridae | |
Low levels of Human Cytomegalovirus Infection in Glioblastoma multiforme associates with patient survival; -a case-control study | |
Cecilia Söderberg-Naucler2  Jan Willems4  Chato Taher2  Inti Peredo1  Abiel Orrego4  Giuseppe Stragliotto3  Afsar Rahbar2  | |
[1] Department of Neurosurgery, Karolinska University Hospital, Stockholm, Sweden;Department of Medicine, Solna, Center for Molecular Medicine, Karolinska Institutet, 171 76Stockholm, Sweden;Department of Neurology , Karolinska University Hospital, Stockholm, Sweden;Department of Pathology, Karolinska University Hospital, Stockholm, Sweden | |
关键词: Survival; Glioblastoma; HCMV; | |
Others : 801229 DOI : 10.1186/2042-4280-3-3 |
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received in 2011-07-08, accepted in 2012-03-16, 发布年份 2012 | |
【 摘 要 】
Background
Glioblastoma multiforme (GBM) represent the most aggressive brain tumor with a median overall survival of about 12-15 months. Over 90% of GBM tumors have recently been shown to be infected with human cytomegalovirus (HCMV). In this case-control study, we evaluated whether there was an association between the grade of HCMV infection and long-term survival (> 18 months) in GBM patients.
Material and methods
Brain tumor tissue sections from consecutive GBMs patients who survived more than 18 months (n = 40), and an equal number of GBM patients, matched to date of diagnosis and surgery, operated at Karolinska University Hospital in 2000-2005 were selected. HCMV infection grade was determined by estimation of the number of HCMV positive cells (scored negative or grade 1-4) in tumor tissue specimens. Using Chi-Square test and logistic regression analysis, we analyzed whether there was an association between long-term survival and HCMV low-grade infection or other clinical parameters known to be associated with prolonged survival of GBM patients; age under 50 years, radical surgery or low recursive partition analysis (RPA) subclass.
Results
HCMV infection was detected in tumor samples from 79 of 80 patients (99%). Among patients surviving > 18 months, HCMV infection grade 1 in the GBM tumor was predominant. A low grade HCMV infection was found in 19 patients, of these 16 survived > 18 months. Thus, 16 of 40 (40%) GBM patients who lived > 18 months had low-grade HCMV infection while only 3 of 40 (8%) GBM patients who lived < 18 months did (p .0006, Chi-Square test). Multiple logistic regression analyses yielded an odds ratio estimate of 6.604 with 95% confidence interval (1.36-32.1) (p .019) for low grade HCMV after adjustment for RPA class III and IV, radical surgery, age and gamma knife treatment.
Conclusion
In conclusion, we found that low-grade HCMV infection was strongly associated with long-term survival in GBM patients.
【 授权许可】
2012 Rahbar et al; licensee BioMed Central Ltd.
【 预 览 】
Files | Size | Format | View |
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20140708004415694.pdf | 860KB | download | |
Figure 1. | 109KB | Image | download |
【 图 表 】
Figure 1.
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