Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro

Head & Face Medicine

Myriam Angélica De la Garza-Ramos⁴  Marcela Márquez-M¹  Carlos Eduardo Medina-De la Garza⁵  Andrés Mendiola-Jiménez⁵  Ricardo Emmanuel Romero-Zavaleta²  Ada Pricila López-Lozano⁴  Yolanda Gutiérrez-Puente³  Casiano Del Angel-Mosqueda⁴ 

[1] Department of Oncology-Pathology, CCK, Karolinska Institutet, Stockholm, Sweden;Unidad de Odontología Integral y Especialidades, Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Nuevo León, Monterrey, México;Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México;Facultad de Odontología, Universidad Autónoma de Nuevo León, Nuevo León, Monterrey, México;Facultad de Medicina, Universidad Autónoma de Nuevo León, Nuevo León, Monterrey, México

关键词: Bone remodelling;    Bone mineralization;    Osteogenic differentiation;    Basic fibroblast growth factor;    Epidermal growth factor;    Dental pulp stem cells;   

Others  :  1224588
DOI  :  10.1186/s13005-015-0086-5

 received in 2015-04-17, accepted in 2015-08-17,  发布年份 2015

【 摘 要 】

Introduction

Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs).

Material and methods

Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR.

Results

EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect.

Conclusion

These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology.

【 授权许可】

   
2015 Del Angel-Mosqueda et al.

【 图 表 】

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