Plasma levels of TNF-α, IFN-γ, IL-4 and IL-10 during a course of experimental contagious bovine pleuropneumonia

BMC Veterinary Research

Joerg Jores¹  Jane Poole¹  Jan Naessens¹  Rossella Lelli²  Attilio Pini²  Massimo Scacchia²  Romolo Salini²  Mirella Luciani²  Flavio Sacchini² 

[1] International Livestock Research Institute, Old Naivasha Road, PO Box 30709, 00100 Nairobi, Kenya;Istituto "G. Caporale", via Campo Boario, 64100 Teramo, Italy

关键词: IL-10;    IL-4;    IFN-γ;    TNF-α;    Cytokines;    Mycoplasma mycoides subsp. mycoides;    Contagious bovine pleuropneumonia;   

Others  :  1119843
DOI  :  10.1186/1746-6148-8-44

 received in 2011-11-29, accepted in 2012-04-25,  发布年份 2012

【 摘 要 】

Background

Contagious Bovine Pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides, is widespread in sub-Saharan Africa. The current live vaccine T1/44 has limited efficacy and occasionally leads to severe side effects in the animals. A better understanding of the immune responses triggered by Mycoplasma mycoides subsp. mycoides and their role in disease progression will help to facilitate the design of a rational vaccine. Currently, knowledge of cytokines involved in immunity and immunopathology in CBPP is rather limited. The aim of this study was to characterize the in vivo plasma concentrations of the cytokines TNF-α, IFN-γ, IL-4, IL-10 and the overall role of CD4⁺ T cells in the development of cytokine levels during a primary infection. Plasma cytokine concentrations in two groups of cattle (CD4⁺ T cell-depleted and non-depleted cattle) experimentally infected with Mycoplasma mycoides subsp. mycoides were measured and their relationship to the clinical outcomes was investigated.

Results

Plasma cytokine concentrations varied between animals in each group. Depletion of CD4⁺ T cells did not induce significant changes in plasma levels of TNF-α, IL-4, and IL-10, suggesting a minor role of CD4⁺ T cells in regulation or production of the three cytokines during the time window of depletion (1-2 weeks post depletion). Unexpectedly, the IFN-γ concentrations were slightly, but statistically significantly higher in the depleted group (p < 0.05) between week three and four post infection. Three CD4⁺ T cell-depleted animals that experienced severe disease, had high levels of TNF-α and IFN-γ. Only one severely diseased non-depleted animal showed a high serum concentration of IL-4 post infection.

Conclusions

Comparison of most severely diseased animals, which had to be euthanized prior to the expected date, versus less severe diseased animals, irrespective of the depletion status, suggested that high TNF-α levels are correlated with more severe pathology in concomitance with high IFN-γ levels.

【 授权许可】

   
2012 Sacchini et al; licensee BioMed Central Ltd.

【 图 表 】

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