Hepatic lipogenesis gene expression in two experimental egg-laying lines divergently selected on residual food consumption

Genetics Selection Evolution

Madeleine Douaire²  André Bordas¹  Stéphanie Daval²  Sandrine Lagarrigue² 

[1] Laboratoire de génétique factorielle, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France;Laboratoire de génétique animale, Institut national de la recherche agronomique, École nationale supérieure agronomique de Rennes, 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France

关键词: liver;    mRNA;    fatness;    food efficiency;    laying fowl;   

Others  :  1094817
DOI  :  10.1186/1297-9686-32-2-205

 received in 1999-09-13, accepted in 1999-12-06,  发布年份 2000

【 摘 要 】

Two Rhode Island Red egg-laying lines have been divergently selected on residual food intake (low intake R^- line, high intake R⁺ line) for 19 generations. In addition to direct response, correlated responses have altered several other traits such as carcass adiposity and lipid contents of several tissues, the R⁺ animals being leaner than the R^- ones. In a search for the biological origin of the differences observed in fat deposit, the hepatic mRNA amounts of genes involved in lipid metabolism were investigated. No difference was found between lines for mRNA levels of ATP citrate-lyase, acetyl-CoA carboxylase, fatty acid synthase, malic enzyme and CCAAT/enhancer binding protein α, a transcription factor acting on several lipogenesis genes. The genes coding for stearoyl-CoA desaturase and apolipoprotein A1 displayed significantly lower mRNA levels in the R⁺ cockerels compared to the R^-. All together these mRNA levels explained 40% of the overall variability of abdominal adipose tissue weight, suggesting an important role of both genes in the fatness variability.

【 授权许可】

   
2000 INRA, EDP Sciences