Gene expression of inflammasome components in peripheral blood mononuclear cells (PBMC) of vascular patients increases with age

Immunity & Ageing

Susanne Dihlmann²  Dittmar Böckler²  Jian Zhang¹  Markus Wortmann²  Maani Hakimi³  Xiaoyu Wu¹ 

[1] Department of Vascular & Thyroid Surgery, The First Hospital of China Medical University, Shenyang, China;Department of Vascular and Endovascular Surgery, University of Heidelberg, Im Neuenheimer Feld 110, Heidelberg, 69120, Germany;Vaskuläre Biomaterialbank Heidelberg, VBBH, Heidelberg, Germany

关键词: Atherosclerosis;    NLRP3;    AIM2;    Aging;    Inflammation;    Vascular disease;   

Others  :  1230079
DOI  :  10.1186/s12979-015-0043-y

 received in 2015-07-27, accepted in 2015-09-23,  发布年份 2015

【 摘 要 】

Background

Chronic low-grade inflammation is considered a driver of many age-related disorders, including vascular diseases (inflammaging). Inhibition of autophagic capacity with ageing was postulated to generate a pro-inflammatory condition via activation of inflammasomes, a group of Interleukin-1 activating intracellular multi-protein complexes. We thus investigated gene expression of inflammasome components in PBMC of 77 vascular patients (age 22–82) in association with age.

Findings

Linear regression of real-time qRT-PCR data revealed a significant positive association of gene expression of each of the inflammasome components with age (Pearson correlation coefficients: AIM2: r = 0.245; P = 0.032; NLRP3: r = 0.367; P = 0.001; ASC (PYCARD): r = 0.252; P = 0.027; CASP1: r = 0.296; P = 0.009; CASP5: r = 0.453; P = 0.00003; IL1B: r = 0.247; P = 0.030). No difference in gene expression of AIM2, NLRP3, ASC CASP1, and CASP5 was detected between PBMC of patients with advanced atherosclerosis and other vascular patients, whereas IL1B expression was increased in PBMC of the latter group (P = 0.0005).

Conclusion

The findings reinforce the systemic pro-inflammatory phenotype reported in elderly by demonstrating an increased phase-1 activation of inflammasomes in PBMC of vascular patients.

【 授权许可】

   
2015 Wu et al.

【 图 表 】

Fig. 1.

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