Gold nanoparticle-labeled biosensor for rapid and sensitive detection of bacterial pathogens

Journal of Biological Engineering

Evangelyn C. Alocilja²  Yun Wang¹ 

[1]Present address: Division of Food Processing Science and Technology, U. S. Food and Drug Administration, Bedford Park 60501, IL, USA

[2]Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing 48824, MI, USA

关键词: Antibodies;    Electrochemical measurement;    Magnetic separation;    Nanoparticles;    Rapid detection;    Biosensor;    E. coli O157:H7;   

Others  :  1230535
DOI  :  10.1186/s13036-015-0014-z

 received in 2015-08-17, accepted in 2015-09-15,  发布年份 2015

【 摘 要 】

Background

Escherichia coli O157:H7 is one of the major foodborne bacterial pathogens and also a biodefense agent. To ensure food safety and public health, it is very important to develop rapid methods for E. coli O157:H7 detection. In this study, we designed a nanoparticle-labeled biosensor for the rapid detection of E. coli O157:H7 in broth.

Results

Magnetic nanoparticles (MNPs) were conjugated with monoclonal antibodies (Abs) to separate target E. coli O157:H7 cells from broth samples. Gold nanoparticles (AuNPs) were conjugated with polyclonal Abs, and were then introduced to the MNP-target complex to form a sandwich MNP-target-AuNP. By measuring the amount of AuNPs through an electrochemical method, the presence and the amount of the target bacteria were determined. Results showed a sensitivity of 10 ¹colony forming units per milliliter (cfu/ml) with a linear range of 10 ¹ –10 ⁶  cfu/ml.

Conclusions

Compared to conventional culture plating methods, the biosensor reduced the detection time from 2 to 4 days to less than 1 hour with a simple target extraction method. The AuNP-labeled biosensor has potential applications in the rapid detection of infectious agents for public health, biodefense, and food/water safety.

【 授权许可】

   
2015 Wang and Alocilja.

【 图 表 】

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