Regulation of MMP-3 expression and secretion by the chemokine eotaxin-1 in human chondrocytes

Journal of Biomedical Science

Chien-Ho Chen⁴  Yung-Feng Lin⁴  Chao-Wen Cheng¹  Ming-Shium Hsieh²  Pin-Zhir Chao³ 

[1] Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan;Department of Orthopedics, En Chu Kong Hospital, New Taipei, Taiwan;Department of Otolaryngology, Taipei Medical University Shuang-Ho Hospital, New Taipei, Taiwan;School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan

关键词: eotaxin-1;    MMP-3;    chondrocyte;    cartilage degradation;    chemokine;    osteoarthritis;   

Others  :  828940
DOI  :  10.1186/1423-0127-18-86

 received in 2011-08-17, accepted in 2011-11-25,  发布年份 2011

【 摘 要 】

Background

Osteoarthritis (OA) is characterized by the degradation of articular cartilage, marked by the breakdown of matrix proteins. Studies demonstrated the involvement of chemokines in this process, and some may potentially serve as diagnostic markers and therapeutic targets; however, the underlying signal transductions are not well understood.

Methods

We investigated the effects of the CC chemokine eotaxin-1 (CCL11) on the matrix metalloproteinase (MMP) expression and secretion in the human chondrocyte cell line SW1353 and primary chondrocytes.

Results

Eotaxin-1 significantly induced MMP-3 mRNA expression in a dose-dependent manner. Inhibitors of extracellular signal-regulated kinase (ERK) and p38 kinase were able to repress eotaxin-1-induced MMP-3 expression. On the contrary, Rp-adenosine-3',5'-cyclic monophosphorothioate (Rp-cAMPs), a competitive cAMP antagonist for cAMP receptors, and H-89, a protein kinase A (PKA) inhibitor, markedly enhanced eotaxin-1-induced MMP-3 expression. These results suggest that MMP-3 expression is specifically mediated by the G protein-coupled eotaxin-1 receptor activities. Interestingly, little amount of MMP-3 protein was detected in the cell lysates of eotaxin-1-treated SW1353 cells, and most of MMP-3 protein was in the culture media. Furthermore we found that the eotaxin-1-dependent MMP-3 protein secretion was regulated by phospholipase C (PLC)-protein kinase C (PKC) cascade and c-Jun N-terminal kinase (JNK)/mitogen-activated protein (MAP) kinase pathways. These data indicate a specific regulation of MMP-3 secretion also by eotaxin-1 receptor activities.

Conclusions

Eotaxin-1 not only induces MMP-3 gene expression but also promotes MMP-3 protein secretion through G protein-coupled eotaxin-1 receptor activities. Chemokines, such as eotaxin-1, could be a potential candidate in the diagnosis and treatment of arthritis.

【 授权许可】

   
2011 Chao et al; licensee BioMed Central Ltd.

【 图 表 】

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