An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

BMC Veterinary Research

François Thiaucourt²  Stéphane Ostrowski⁹  Ali Madad Rajabi⁸  Hafizullah Noori⁸  Willy Schauwers³  Ghulam Mohammad Ziay^1,10  Mohibullah Halimi^1,10  Nadia Mukhtar⁵  Muhammad Zubair Shabbir⁵  Tahir Yaqub⁵  Mahmad Reshad Jaumally¹  Deodass Meenowa¹  Eric Cardinale⁴  Martha Yami^1,11  Shiferaw Jenberie^1,11  Isabel Gacheri Nkando⁷  Hezron Wesonga⁷  Charles Bodjo^1,12  Karim Tounkara^1,12  Mullojon Amirbekov⁶  Tillo Tilloev⁶  Karomatullo Hamroev⁶  Lucía Manso-Silván²  Florence Tardy^1,13  François Poumarat^1,13  Armelle Peyraud² 

[1] Ministère de l”Agriculture, de la technologie alimentaire et des ressources naturelles, A. de Plevitz street Long Mountain Réduit, Maurice, France;INRA, UMR1309 CMAEE, Montpellier F-34398, France;Animal Health and Development Program, Phase II (EU project), Ministry of Agriculture, Jamal Mina, Kabul, Afghanistan;CIRAD UMR CMAEE, Centre de Recherche et de Veille de l”Océan Indien, Sainte-Clotilde 97491 Réunion, France;Quality Operations Laboratory, University of Veterinary and Animal Sciences, Lahore 54600, Pakistan;State Veterinary Inspection Services, Ministry of Agriculture, Rudaki Av 44, Dushanbe 734025, Tajikistan;Kenya Agricultural Research Institute (KARI), Veterinary Research Centre, KARI Muguga North, P.O. Box 32–00902, Kikuyu, Kenya;Wildlife Conservation Society, Afghanistan Program, Kabul, Afghanistan;Wildlife Conservation Society, New York, USA;Animal Health and Production, Ministry of Agriculture, Jamal Mina, Kabul, Afghanistan;National Veterinary Institute, P.O. Box 19, Debre-Zeit, Ethiopia;Pan African Veterinary Vaccine Center (AU-PANVAC), P.O. Box 1746, Debre-Zeit, Ethiopia;Université de Lyon, VetAgro, 1 av Bourgelat Marcy l”étoile, Lyon 69280, France

关键词: Vaccine quality control;    Pakistan;    Afghanistan;    Tajikistan;    Mauritius;    Ethiopia;    Kenya;    Seroprevalence;    Competitive ELISA;    Contagious caprine pleuropneumonia;   

Others  :  1119322
DOI  :  10.1186/1746-6148-10-48

 received in 2013-07-03, accepted in 2014-02-14,  发布年份 2014

【 摘 要 】

Background

Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species of the “mycoides cluster” frequently infecting goat herds. The lack of a more specific test has been a real obstacle to the evaluation of the prevalence and economic impact of CCPP worldwide. A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted at CIRAD and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study.

Results

The strict specificity of the test was confirmed in CCPP-free goat herds exposed to other mycoplasma species of the “mycoides cluster”. Prevalence studies were performed across the enzootic range of the disease in Africa and Asia. Seroprevalence was estimated at 14.6% in the Afar region of Ethiopia, whereas all the herds presented for CCPP vaccination in Kenya tested positive (individual seroprevalence varied from 6 to 90% within each herd). In Mauritius, where CCPP emerged in 2009, nine of 62 herds tested positive. In Central Asia, where the disease was confirmed only recently, no positive animals were detected in the Wakhan District of Afghanistan or across the border in neighboring areas of Tajikistan, whereas seroprevalence varied between 2.7% and 44.2% in the other districts investigated and in northern Pakistan. The test was also used to monitor seroconversion in vaccinated animals.

Conclusions

This newly formatted CCPP cELISA kit has retained the high specificity of the original kit. It can therefore be used to evaluate the prevalence of CCPP in countries or regions without vaccination programs. It could also be used to monitor the efficacy of vaccination campaigns as high-quality vaccines induce high rates of seroconversion.

【 授权许可】

   
2014 Peyraud et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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