Systemically administered liposome-encapsulated Ad-PEDF potentiates the anti-cancer effects in mouse lung metastasis melanoma

Journal of Translational Medicine

Li Yang²  Bing Kan²  Yong-qiu Mao²  Lian Lu²  Hai-long Zhang²  Shun-tao Luo²  Meng Li²  Xiao-peng Li²  Xiao-qing Su²  Wei Wei²  Li-ping Yang¹  Hua-shan Shi³ 

[1] Department of Oncology, The First Hospital of Lanzhou University, Lanzhou, Gansu, PR China;State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Clinical Medicine School, Sichuan University, Chengdu, Sichuan, PR China;State Key Laboratory of Biotherapy and Department of Head and Neck Oncology, West China Hospital, West China Medical School, Sichuan University, Keyuan Road 4, Chengdu, Sichuan, PR China

关键词: Gene therapy;    Melanoma;    Cationic liposome;    Adenovirus;    PEDF;   

Others  :  827826
DOI  :  10.1186/1479-5876-11-86

 received in 2012-11-11, accepted in 2013-03-14,  发布年份 2013

【 摘 要 】

Background

The use of adenoviral vector for gene therapy is still an important strategy for advanced cancers, however, the lack of the requisite coxsackie-adenovirus receptor in cancer cells and host immune response to adenovirus limit the application of adenoviral vector in vivo.

Method

We designed the antiangiogenic gene therapy with recombinant PEDF adenovirus (Ad-PEDF) encapsulated in cationic liposome (Ad-PEDF/Liposome), and investigated the anti-tumor efficacy of Ad-PEDF/Liposome complex on inhibition of tumor metastasis.

Results

We found that systemic administration of Ad-PEDF/liposome was well tolerated and resulted in marked suppression of tumor growth, and was more potent than uncoated Ad-PEDF to induce apoptosis in B16-F10 melanoma cells and inhibit murine pulmonary metastases in vivo. After Ad-luciferase was encapsulated with liposome, its distribution decreased in liver and increased in lung. The anti-Ad IgG level of Ad-PEDF/Liposome was significantly lower than Ad-PEDF used alone.

Conclusion

The present findings provide evidences of systematic administration of cationic liposome-encapsulated Ad-PEDF in pulmonary metastatic melanoma mice model, and show an encouraging therapeutic effect for further exploration and application of more complexes based on liposome-encapsulated adenovirus for more cancers.

【 授权许可】

   
2013 Shi et al.; licensee BioMed Central Ltd.

【 图 表 】

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