Existence of a potential neurogenic system in the adult human brain

Journal of Translational Medicine

Manoel Jacobsen Teixeira⁵  Paulo Eurípedes Marchiori¹  Ariel Barreto Nogueira³  Sheila Aparecida Siqueira³  Alison Colquhoun⁴  Mari Cleide Sogayar²  Adriano Barreto Nogueira⁵ 

[1] Department of Neurology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil;Cell and Molecular Therapy Center, Faculty of Medicine, University of São Paulo, São Paulo, Brazil;Department of Pathology, Hospital das Clínicas, Faculty of Medicine, University of São Paulo, São Paulo, Brazil;Department of Cellular and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil;Laboratory of Experimental Surgery Research, Hospital das Clínicas, Faculty of Medicine, University of São Paulo, Avenida Dr. Eneas de Carvalho Aguiar 255, 05403-900 São Paulo, Brazil

关键词: Doublecortin;    Nestin;    Hypothalamus;    Hippocampus;    Temporal lobe;    Limbic system;    Adult human brain;    Neural stem cell;    Neurogenesis;    Neurogenic niche;   

Others  :  1148923
DOI  :  10.1186/1479-5876-12-75

 received in 2013-12-25, accepted in 2014-03-13,  发布年份 2014

【 摘 要 】

Background

Prevailingly, adult mammalian neurogenesis is thought to occur in discrete, separate locations known as neurogenic niches that are best characterized in the subgranular zone (SGZ) of the dentate gyrus and in the subventricular zone (SVZ). The existence of adult human neurogenic niches is controversial.

Methods

The existence of neurogenic niches was investigated with neurogenesis marker immunostaining in histologically normal human brains obtained from autopsies. Twenty-eight adult temporal lobes, specimens from limbic structures and the hypothalamus of one newborn and one adult were examined.

Results

The neural stem cell marker nestin stained circumventricular organ cells and the immature neuronal marker doublecortin (DCX) stained hypothalamic and limbic structures adjacent to circumventricular organs; both markers stained a continuous structure running from the hypothalamus to the hippocampus. The cell proliferation marker Ki-67 was detected predominately in structures that form the septo-hypothalamic continuum. Nestin-expressing cells were located in the fimbria-fornix at the insertion of the choroid plexus; ependymal cells in this structure expressed the putative neural stem cell marker CD133. From the choroidal fissure in the temporal lobe, a nestin-positive cell layer spread throughout the SVZ and subpial zone. In the subpial zone, a branch of this layer reached the hippocampal sulcus and ended in the SGZ (principally in the newborn) and in the subiculum (principally in the adults). Another branch of the nestin-positive cell layer in the subpial zone returned to the optic chiasm. DCX staining was detected in the periventricular and middle hypothalamus and more densely from the mammillary body to the subiculum through the fimbria-fornix, thus running through the principal neuronal pathway from the hippocampus to the hypothalamus. The column of the fornix forms part of this pathway and appears to coincide with the zone previously identified as the human rostral migratory stream. Partial co-labeling with DCX and the neuronal marker βIII-tubulin was also observed.

Conclusions

Collectively, these findings suggest the existence of an adult human neurogenic system that rises from the circumventricular organs and follows, at minimum, the circuitry of the hypothalamus and limbic system.

【 授权许可】

   
2014 Nogueira et al.; licensee BioMed Central Ltd.

【 图 表 】

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