Association between miR-27a genetic variants and susceptibility to colorectal cancer

Diagnostic Pathology

Sanyuan Hu⁴  Yuanjie Xuan³  Xiaofang Liu²  Yeli Wang³  Xiaoli Sun¹  Zaiqiu Wang³ 

[1] Department of Laboratory, Yuhuangding Hospital, Yantai, Shandong 264000, China;Department of Hepatobiliary, Yuhuangding Hospital, Yantai, Shandong 264000, China;Department of Anorectal, Yuhuangding Hospital, Yantai, Shandong 264000, China;Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China

关键词: Risk factor;    miR-27a;    Colorectal cancer;    Single nucleotide polymorphism;   

Others  :  1150027
DOI  :  10.1186/1746-1596-9-146

 received in 2014-03-25, accepted in 2014-06-13,  发布年份 2014

【 摘 要 】

Background

MicroRNAs (miRNAs) are short, non-coding RNAs that negatively regulate target genes. A single nucleotide polymorphism (SNP) in a miRNA sequence may alter miRNA expression and/or maturation, which was proposed to associate with the development and progression of cancer. The rs895819 polymorphism, located in the terminal loop of pre-miR-27a, has been reported to have relevance to several cancers. In this study, we investigated the possibility of association between polymorphism in rs895819 and susceptibility to colorectal cancer (CRC).

Methods

We identified a single SNP, rs895819 in pre-miR-27a, for further investigation, were determined in 205 CRC patients and 455 healthy controls.

Results

When taking the AA genotype as a reference, we found that AG genotype was not statistically significantly associated with the risk of CRC (AG vs. AA, OR 1.245, 95% CI: 0.806 – 1.923). However, the GG genotype was significantly associated with risk of CRC (GG vs. AA, OR 1.599, 95% CI: 1.052 – 2.430). In the AG + GG vs GG group, no significant difference was detected (OR 1.424, 95% CI, 0.974 – 1.801). GG genotype and G allele was associated with an increased risk of metastasis in this study (P < 0.001 and P = 0.003, respectively).

Conclusions

This study found significant association between rs895819 polymorphism in pre-miR-27a and CRC risk. Population-based studies with large number of subjects and long-term follow-up are needed to verify the association of miR-27a polymorphism with CRC susceptibility and severity.

【 授权许可】

   
2014 Wang et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Fan NJ, Kang R, Ge XY, Li M, Liu Y, Chen HM, Gao CF: Identification alpha-2-HS-glycoprotein precursor and tubulin beta chain as serology diagnosis biomarker of colorectal cancer. Diagn Pathol 2014, 9:53.
• [2]Chen C, Wang L, Liao Q, Huang Y, Ye H, Chen F, Xu L, Ye M, Duan S: Hypermethylation of EDNRB promoter contributes to the risk of colorectal cancer. Diagn Pathol 2013, 8:199.
• [3]Schmoll HJ, Stein A: Colorectal cancer in 2013: Towards improved drugs, combinations and patient selection. Nat Rev Clin Oncol 2014, 11:79-80.
• [4]Yatabe J, Yatabe MS, Ishibashi K, Nozawa Y, Sanada H: Early detection of colon cancer by amino acid profiling using AminoIndex Technology: a case report. Diagn Pathol 2013, 8:203.
• [5]Wang L, Cai S, Teng Z, Zhao X, Chen X, Bai X: Insulin therapy contributes to the increased risk of colorectal cancer in diabetes patients: a meta-analysis. Diagn Pathol 2013, 8:180.
• [6]Liu F, Xiong Y, Zhao Y, Tao L, Zhang Z, Zhang H, Liu Y, Feng G, Li B, He L, Ma J, Qin S, Yang Y: Identification of aberrant microRNA expression pattern in pediatric gliomas by microarray. Diagn Pathol 2013, 8:158.
• [7]Wang S, Li H, Wang J, Wang D: Expression of microRNA-497 and its prognostic significance in human breast cancer. Diagn Pathol 2013, 8:172.
• [8]Qin W, Zhang K, Clarke K, Weiland T, Sauter ER: Methylation and miRNA Effects of Resveratrol on Mammary Tumors vs Normal Tissue. Nutr Cancer 2014, 66:270-277.
• [9]Zhou J, Yu L, Gao X, Hu J, Wang J, Dai Z, Wang JF, Zhang Z, Lu S, Huang X, Wang Z, Qiu S, Wang X, Yang G, Sun H, Tang Z, Wu Y, Zhu H, Fan J: Plasma microRNA panel to diagnose hepatitis B virus-related hepatocellular carcinoma. J Clin Oncol 2011, 29:4781-4788.
• [10]Tang W, Zhu J, Su S, Wu W, Liu Q, Su F, Yu F: MiR-27 as a prognostic marker for breast cancer progression and patient survival. PLoS One 2012, 7:e51702.
• [11]Jahid S, Sun J, Edwards RA, Dizon D, Panarelli NC, Milsom JW, Sikandar SS, Gümüs ZH, Lipkin SM: miR-23a promotes the transition from indolent to invasive colorectal cancer. Cancer Discov 2012, 2:540-553.
• [12]Kupcinskas J, Wex T, Link A, Leja M, Bruzaite I, Steponaitiene R, Juzenas S, Gyvyte U, Ivanauskas A, Ancans G, Petrenkiene V, Skieceviciene J, Kupcinskas L, Malfertheiner P: Gene polymorphisms of micrornas in Helicobacter pylori-induced high risk atrophic gastritis and gastric cancer. PLoS One 2014, 9:e87467.
• [13]Catucci I, Verderio P, Pizzamiglio S, Bernard L, Dall'olio V, Sardella D, Ravagnani F, Galastri L, Barile M, Peissel B, Zaffaroni D, Manoukian S, Radice P, Peterlongo P: The SNP rs895819 in miR-27a is not associated with familial breast cancer risk in Italians. Breast Cancer Res Treat 2012, 133:805-807.
• [14]Yang R, Schlehe B, Hemminki K, Sutter C, Bugert P, Wappenschmidt B, Volkmann J, Varon R, Weber BH, Niederacher D, Arnold N, Meindl A, Bartram CR, Schmutzler RK, Burwinkel B: A genetic variant in the pre-miR-27a oncogene is associated with a reduced familial breast cancer risk. Breast Cancer Res Treat 2010, 121:693-702.
• [15]Sun Q, Gu H, Zeng Y, Xia Y, Wang Y, Jing Y, Yang L, Wang B: Hsa-mir-27a genetic variant contributes to gastric cancer susceptibility through affecting miR-27a and target gene expression. Cancer Sci 2010, 101:2241-2247.
• [16]Fu J, Tang W, Du P, Wang G, Chen W, Li J, Zhu Y, Gao J, Cui L: Identifying microRNA-mRNA regulatory network in colorectal cancer by a combination of expression profile and bioinformatics analysis. BMC Syst Biol 2012, 6:68.
• [17]Kim TD, Lee SU, Yun S, Sun HN, Lee SH, Kim JW, Kim HM, Park SK, Lee CW, Yoon SR, Greenberg PD, Choi I: Human microRNA-27a* targets Prf1 and GzmB expression to regulate NK-cell cytotoxicity. Blood 2011, 118:5476-5486.
• [18]Del Follo-Martinez A, Banerjee N, Li X, Safe S, Mertens-Talcott S: Resveratrol and quercetin in combination have anticancer activity in colon cancer cells and repress oncogenic microRNA-27a. Nutr Cancer 2013, 65:494-504.
• [19]Zhou Y, Du WD, Chen G, Ruan J, Xu S, Zhou FS, Zuo XB, Lv ZJ, Zhang XJ: Association analysis of genetic variants in microRNA networks and gastric cancer risk in a Chinese Han population. J Cancer Res Clin Oncol 2012, 138:939-945.
• [20]Wang B, Ma N, Wang Y: Association between the hsa-mir-27a variant and breast cancer risk: a meta-analysis. Asian Pac J Cancer Prev 2012, 13:6207-6210.
• [21]Hezova R, Kovarikova A, Bienertova-Vasku J, Sachlova M, Redova M, Vasku A, Svoboda M, Radova L, Kiss I, Vyzula R, Slaby O: Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer. World J Gastroenterol 2012, 18:2827-2831.