Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet

Diabetology & Metabolic Syndrome

Séverine Sigrist¹  Valérie Schini-Kerth³  Elisa Maillard-Pedracini¹  Nathalie Jeandidier²  Michel Pinget²  William Bietiger¹  Stéphanie Dal¹  Nathalie Auberval¹ 

[1] UMR DIATHEC, EA 7294, Centre Européen d’Etude du Diabète, Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Bld René Leriche, 67200 Strasbourg, France;Structure d’Endocrinologie, Diabète –Nutrition et Addictologie, Pôle NUDE, Hôpitaux Universitaires de Strasbourg, (HUS), 67000 Strasbourg, France;Département de Pharmacologie et Physicochimie, UMR 7213 Centre National de la Recherche Scientifique, Université de Strasbourg, Faculté de Pharmacie, BP60024, 67401 Illkirch, France

关键词: Complications;    Oxidative stress;    High-fat diet;    Metabolic syndrome;   

Others  :  1136520
DOI  :  10.1186/1758-5996-6-130

 received in 2014-05-18, accepted in 2014-09-29,  发布年份 2014

【 摘 要 】

Background

Metabolic syndrome is associated with an increased risk of cardiovascular and hepatic complications. Oxidative stress in metabolic tissues has emerged as a universal feature of metabolic syndrome and its co-morbidities. We aimed to develop a rapidly and easily induced model of metabolic syndrome in rats to evaluate its impact on plasma and tissue oxidative stress.

Materials and methods

Metabolic syndrome was induced in rats using a high-fat diet (HFD), and these rats were compared to rats fed a normal diet (ND) for 2 months. Metabolic control was determined by measuring body weight, blood glucose, triglycerides, lipid peroxidation and protein carbonylation in plasma. Insulinemia was evaluated through the measure of C-peptide. Histological analysis was performed on the pancreas, liver and blood vessels.

Results

After 2 months, the HFD induced an increase in body weight, insulin and triglycerides. Liver steatosis was also observed in the HFD group, which was associated with an increase in glycogen storage. In the pancreas, the HFD induced islet hyperplasia. Tissue oxidative stress was also increased in the liver, pancreas and blood vessels, but plasma oxidative stress remained unchanged.

Conclusion

This paper reports the development of a fast and easy model of rat metabolic syndrome associated with tissue oxidative stress. This model may be a good tool for the biological validation of drugs or antioxidants to limit or prevent the complications of metabolic syndrome.

【 授权许可】

   
2014 Auberval et al.; licensee BioMed Central Ltd.

【 图 表 】

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