Journal of Biomedical Science | |
Detection of vascular endothelial growth factor in colon cancer xenografts using bevacizumab based near infrared fluorophore conjugate | |
Keigo Endo2  Yoshito Tsushima1  Hideyuki Tominaga1  Dilip Shah3  Pramila Paudyal1  Bishnuhari Paudyal1  | |
[1] Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan;Kyoto College of Medical Science, Nantan, Kyoto 6220041, Japan;Center for Translational Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA | |
关键词: Colorectal cancer; Bevacizumab; Optical Imaging; VEGF; Near infrared fluorescence; | |
Others : 817663 DOI : 10.1186/1423-0127-21-35 |
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received in 2014-03-10, accepted in 2014-04-23, 发布年份 2014 | |
【 摘 要 】
Background
The aim of this study was to develop the near infrared fluorescence (NIRF)-based imaging agent for the visualization of vascular endothelial growth factor (VEGF) in colon cancer. AlexaFluor 750 conjugating with bevacizumab, and injected intravenously into nude mice bearing VEGF over-expressing HT29 human colorectal cancer. Optical imaging was performed at 15 min, 24 h and 48 h post injection. Immunofluorescences staining of the tumor sections were performed. HT29 colorectal cancer xenografts were clearly visualized with bevacizumab-AlexaFluor 750.
Results
Ex vivo analysis showed 2.1 ± 0.4%, 37.6 ± 6.3% and 38.5 ± 6.2% injected dose/g accumulated in the tumors at 15 min, 24 h and 48 h respectively. Tumor uptake was significantly decreased in pretreated with excess of bevacizumab (p = 0.002). Immunofluorescence analysis showed strong staining of anti-CD 31 antibody around the blood vessels. Anti-VEGF-A and bevacizumab showed heterogeneous expression throughout the tumor.
Conclusions
Current study successfully detected the VEGF expression in HT29 colorectal cancer xenografts, signifying as a potential agent for non-invasive imaging of VEGF expression, which may be applied in clinical practice.
【 授权许可】
2014 Paudyal et al.; licensee BioMed Central Ltd.
【 预 览 】
Files | Size | Format | View |
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20140711014115834.pdf | 1048KB | download | |
Figure 5. | 19KB | Image | download |
Figure 4. | 59KB | Image | download |
Figure 3. | 33KB | Image | download |
Figure 2. | 46KB | Image | download |
Figure 1. | 95KB | Image | download |
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