Influence of bioaugmentation on biodegradation of phenanthrene-contaminated soil by earthworm in lab scale

Journal of Environmental Health Science Engineering

Yousef Dadban Shahamat¹  Ali Esrafili³  Kamyar Yaghmaeian²  Amirhossein Mahvi²  Simin Nasseri²  Roshanak Rezaei Kalantary³  Ahmad Jonidi Jafari³  Hosseinali Asgharnia³ 

[1] Department of Environmental Health Engineering, School of Public Health, Golestan University of Medical Sciences, Gorgan, Iran;Center for Water Quality Research, Institute of Environmental Research, Tehran University of Medical Sciences, Tehran, Iran;Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran

关键词: Earthworm and bacteria;    Phenanthrene;    Biodegradation;    Bioaugmentation;   

Others  :  1133716
DOI  :  10.1186/s40201-014-0150-2

 received in 2014-07-26, accepted in 2014-12-14,  发布年份 2014

【 摘 要 】

Background

Use of earthworm to eliminate the phenanthrene from the soil (bioaccumulation) is developed as an economical method. Bioaugmentation of microorganism was used for promotion of bioaccumulation by earthworm. The aim of this study was to determine the bioaccumulation or biodegradation of phenanthrene by Eisenia fetida and bacterial consortium in polluted soil.

Methods

The amount of 0.4 kg of the polluted soil in the ratio of 10 and 30 mg phenanthrene per kg of dry soil was transferred into each pot. Afterwards, bacteria and earthworms were added to each pot in separate and combination. The samples were kept under field conditions, and the retention concentrations of phenanthrene were analyzed after 8 weeks.

Results

Results showed that the Eisenia fetida was able to significantly remove phenanthrene from the polluted soil samples. Bioaccumulation and bioaugmentation alone have the removal efficiency of 60.24% and 50.3%, respectively. In the combined mode, phenanthrene removal efficiency was 63.81%.

Conclusions

The current study indicated that the use of earthworms, could improve both phenanthrene bioavailability and microbial activity, which led to enhancing removal of carbon-based pollutants.

【 授权许可】

   
2014 Asgharnia et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Karyab H, Yunesian M, Nasseri S, Mahvi AH, Ahmadkhaniha R, Rastkari N: Polycyclic Aromatic Hydrocarbons in drinking water of Tehran, Iran. J Environ Health Sci Eng 2013, 11(25).
• [2]Kalantary RR, Badkoubi A, Mohseni-Bandpi A, Esrafili A, Baneshi MM, Jorfi S: Modification of phenanthrenes biodegradation with Humic compounds. Soil Sed Cont 2013, 22:185-198.
• [3]Karyab H, Rastkari N, Nabizadeh R, Nasseri S, Ahmadkhaniha R, Mahvi AH: Determination and source identification of polycyclic aromatics hydrocarbons in Karaj River, Iran. Bull Environ Contam Toxicol 2014, 92:50-56.
• [4]Hernández-Castellanos B, Ortíz-Ceballos A, Martínez-Hernández S, Noa-Carrazana JC, Luna-Guido M, Dendooven L: Removal of benzo (a) pyrene from soil using an endogeic earthworm Pontoscolex corethrurus (Müller, 1857).Appl Soil Eco 2013, 9:62–70.
• [5]Alvarez PJ, Illman W: Bioremediation and natural attenuation: process fundamentals and mathematical models.John Wiley & Sons; 2005.
• [6]Scullion J: Remediating polluted soils. Naturwissenschaften 2006, 93(2):345-356.
• [7]Merkl N, Kraft R, Arias M: Effect of the tropical grass Brachiaria brizantha (Hochst. ex A. Rich.) stap on microbial population and activity in petroleum contaminated soil. Microbiol Res 2006, 161:80-91.
• [8]Lundstedt S: Analysis of PAHs and their Transformation Product in Contaminated Soil and Remedial Processes. Department of Chemistry Environmental Chemistry, Umeå University, SWEDEN; 2003.
• [9]Hidayat A, Tachibana S: Biodegradation of aliphatic hydrocarbon in three types of crude oil by Fusarium sp. F092 under stress with artificial sea water. J Environ Sci Technol 2012, 5(1):64-73.
• [10]Wu S, Wu E, Qiu L, Zhong W, Chen J: Effects of phenanthrene on the mortality, growth, and anti-oxidant system of earthworms (Eisenia fetida) under laboratory conditions. Chemosphere 2011, 83:429-434.
• [11]Yan Z, Jiang H, Li X, Shi Y: Accelerated removal of pyrene and benzo[a]pyrene in freshwater sediments with amendment of cyanobacteria-derived organic matter. J Hazard Mater 2014, 272:66-74.
• [12]Mrozik A, Piotrowska-seget Z, Labuzek S: Bacterial degradation and bioremediation of Polycyclic Aromatic Hydrocarbon.Pol J Chem 2003, 12(1):15–25.
• [13]Shafiee P, Shojaosadati SA, Charkhabi AH: Biodegradation of polycyclic aromatic hydrocarbons by aerobic mixed bacterial culture isolated from hydrocarbon polluted soils. Iran J Chem Eng 2006, 25(3):73-78.
• [14]Sinha RK, Bharambe G, Ryan D: Converting wasteland into wonderland by earthworms—a low-cost nature’s technology for soil remediation: a case study of vermiremediation of phenanthrenes contaminated soil. Environmentalist 2008, 28(4):466-475.
• [15]Silvia M, Contreras-Ramos , Alvarez-Bernal D, Dendooven L: Characteristics of earthworms (Eisenia fetida) in phenanthrenes contaminated soil amended with sewage sludge or vermicompost. Appl Soil Ecol 2009, 41:269-276.
• [16]Contreras-Ramos SM, Alvarez-Bernal D, Dendooven L: Removal of polycyclic aromatic hydrocarbons from soil amended with biosolid or vermicompost in the presence of earthworms (Eisenia fetida). Soil Biol Biochem 2008, 40:1954-1959.
• [17]Gajalakshmi S, Abbasi S: Earthworms and vermicomposting. Indian J Biotechnol 2004, 3(4):486-494.
• [18]Dabke SV: Vermi-remediation of heavy metal-contaminated soil. Blacksmith Inst J Health Pollut 2013, 3(4):4-10.
• [19]Belfroid A, Meiling J, Drenth H-J, Hermens J, Seinen W, Vangestel K: Dietary uptake of superlipophilic compounds by earthworms (Eisenia andrei). Ecotox Environ Safe 1995, 31(3):185-191.
• [20]Monard C, Martin-Laurent F, Vecchiato C, Francez AJ, Vandenkoornhuyse P, Binet F: Combined effect of bioaugmentation and bioturbation on atrazine degradation in soil. Soil Biol Biochem 2008, 40:2253-2259.
• [21]Butenschoen O, Ji R, Schaffer A, Scheu S: The fate of catechol in soil as affected by earthworms and clay. Soil Biol Biochem 2009, 41:330-339.
• [22]Yuliani H, Sahlan M, Hermansyah H, Wijanarko A: Selection and identification of polyaromatic hydrocarbon degrading bacteria. World Appl Sci J 2012, 20(8):1133-1138.
• [23]Seo J-S, Keum Y-S, Li QX: Bacterial degradation of aromatic compounds. Int J EnvironRes Public Health 2009, 6:278-309.
• [24]Guo C, Dang Z, Wong Y, Tam NF: Biodegradation ability and dioxgenase genes of phenanthrene-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments. Int Biodeterior Biodegrad 2010, 64:419-426.
• [25]Hu M, Chen H, Tian L, Hu F, Wei Z-G, Li H-X: Role of earthworm in degradation of soil phenanthrene by Pseudomonas putida. Ying Yong Sheng Tai Xue Bao 2008, 19(1):218-222.
• [26]Sun H, Li J, Wang C, Wang L, Wang Y: Enhanced microbial removal of pyrene in soils in the presence of earthworms. Soil Sedi Cont 2011, 20:617-630.
• [27]Nasseri S, Kalantary RR, Nourieh N, Naddafi K, Mahvi AH, Baradaran N: Influence of bioaugmentation in biodegradation of Phenanthrenes-contaminated soil in bio-slurry phase reactor. Iran J Environ Health Sci Eng 2010, 1(3):199-208.
• [28]Baneshi MM, Kalantary RR, Jafari AJ, Nasseri S, Jaafarzadeh N, Esrafili A: Effect of bioaugmentation to enhance phytoremediation for removal of phenanthrene and pyrene from soil with Sorghum and Onobrychis sativa.J Environ Health Sci Eng 2014, 12(24).
• [29]Yu K, Wong A, Yau K, Wong Y, Tam N: Natural attenuation, biostimulation and bioaugmentation on biodegradation of polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments.Marine pollution bulletin 2005; 51:1071–1077.
• [30]Lee S, Lee W, Lee C, Kim J: Degradation of phenanthrene and pyrene in rhizosphere of grasses and legumes. J Hazard Mater 2008, 153(1–2):892-898.
• [31]Sheng-wang P, Shi-qiang W, Xin Y, Sheng-xian C: The removal and remediation of phenanthrene and pyrene in soil by mixed cropping of alfalfa and rape. Agric Sci China 2008, 7(11):1355-1364.
• [32]Peng S, Zhou Q, Cai Z, Zhang Z, Bhorgin AJ: Phytoremediation of petroleum contaminated soils by Mirabilis Jalapa L. in agreenhouse plot experiment. J Hazard Mater 2009, 168:1490-1496.
• [33]Kelsey JW, Slizovskiy IB, Peters RD, Melnick AM: Sterilization affects soil organic matter chemistry and bioaccumulationof spiked p,p-DDE and anthracene by earthworms. Environ Pollut 2010, 158:2251-2257.
• [34]Protection USE, Agency OoSW: Polycyclic Aromatic Hydrocarbons (phenanthrenes). EPA, Washington, DC; 2008.
• [35]Hickman ZA, Reid BJ: Earthworm assisted bioremediation of organic contaminants. Environ Int 2008, 34:1072-1081.
• [36]Greer LE, Shelton DR: Effect of inoculant strain and organic matter content on kinetics of 2,4-dichlorophenoxyacetic acid degradation in soil. Appl Environ Microboil 1992, 58(5):1459-1465.
• [37]Hunter RD, Ekunwe SIN, Dodor DE, Hwang H-M, Ekunwe L: Bacillus subtilis is a potential degrader of pyrene and benzo[a]pyrene. Int J Environ Res Public Health 2005, 2(2):267-271.
• [38]Bhorgin AJ, Uma K: Antimicrobial activity of Earthworm Powder (Lampito mauritii). IntJCurrMicrobiolAppSci 2014, 3(1):437-443.
• [39]Laverty G, Gorman SP, Gilmore BF: The potential of antimicrobial peptides as biocides. Int J Mol Sci 2011, 12(10):6566-6596.
• [40]Wolters V: Invertebrate control of soil organic matter solubility. Soil Biol Biochem 2000, 31:1-19.
• [41]Schaefer M, Juliane F: The influence of earthworms and organic additives on the biodegradation of oil contaminated soil. Appl Soil Ecol 2007, 36(1):53-62.
• [42]Barré P, McKenzie BM, Hallett PD: Earthworms bring compacted and loose soil to a similar mechanical state. Soil Biol Biochem 2009, 41(3):656-658.