Functional and molecular characterisation of mammary side population cells

Breast Cancer Research

Matthew J Smalley²  Trevor C Dale³  Maria d M Vivanco⁴  Alan Ashworth⁴  Tariq Enver¹  Chirag Joshi¹  Helen Clayton⁴  Azra J Alvi⁴ 

[1] Section of Gene Function and Regulation, Institute of Cancer Research, London, UK;Current address: Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam, The Netherlands;Section of Cell and Molecular Biology, Institute of Cancer Research, London, UK;Breakthrough Toby Robins Breast Cancer Centre, Institute of Cancer Research, London, UK

关键词: telomerase;    stem cells;    side population;    differentiation markers;    breast epithelium;   

Others  :  1118817
DOI  :  10.1186/bcr547

 received in 2002-03-20, accepted in 2002-09-18,  发布年份 2002

【 摘 要 】

Background

Breast cancer is thought to arise in mammary epithelial stem cells. However, the identity of these stem cells is unknown.

Methods

Studies in the haematopoetic and muscle systems show that stem cells have the ability to efflux the dye Hoechst 33342. Cells with this phenotype are referred to as the side population (SP). We have adapted the techniques from the haematopoetic and muscle systems to look for a mammary epithelial SP.

Results

Of mammary epithelial cells isolated from both the human and mouse mammary epithelia, 0.2–0.45% formed a distinct SP. The SP was relatively undifferentiated but grew as typical differentiated epithelial clones when cultured. Transplantation of murine SP cells at limiting dilution into cleared mammary fat pads generated epithelial ductal and lobuloalveolar structures.

Conclusion

These data demonstrate the existence of an undifferentiated SP in human and murine mammary epithelium. Purified SP cells are a live single-cell population that retain the ability to differentiate in vitro and in vivo. Studies of haematopoetic cells have suggested that the SP phenotype constitutes a universal stem cell marker. This work therefore has implications for mammary stem cell biology.

【 授权许可】

   
2002 Alvi et al., licensee BioMed Central Ltd

【 图 表 】

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